Anti-Human/Mouse/Rat Glycophorin A – Biotin
Anti-Human/Mouse/Rat Glycophorin A – Biotin
Product No.: G212
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Clone JC159 Target Glycophorin A Formats AvailableView All Alternate Names CD235a, GYPA, GPA Isotype Mouse IgG1 κ Applications FC , IF , IHC , IHC FFPE , WB |
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Antibody DetailsProduct DetailsReactive Species Human/Mouse/Rat Host Species Mouse Immunogen Membrane preparation from splenic hairy cell leukemia Product Concentration 0.5 mg/ml Formulation This Biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Storage and Handling This biotinylated antibody is stable when stored at 2-8°C. Do not freeze. Country of Origin USA Additional Applications Reported In Literature ? WB, IHC, IF, FC Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity JC159 activity is directed against human, mouse, and rat glycophorin A and strongly
labels normal erythroid cells at all stages of differentiation from precursor normoblasts to mature
red blood cells. In erythroleukemia, blast cells are strongly labeled. JC159 does not react with
glycophorin B. Background Glycophorins are transmembrane proteins of red blood cells (RBC)1 . There are four glycophorin encoding genes (A, B, C, and D), and a fifth pseudogene (E), which has no protein product.
Glycophorin A, B, and E are tandemly arranged repeats on chromosome 4 and are prone to
rearrangements that lead to rare blood groups. Glycophorin A and B carry MN and Ss blood group antigens. Glycophorin A is a type I glycoprotein carrying sialylated O- and N-glycans, and most of the O-glycans are NeuAcα2-3Galβ1-3(NeuAcα2-6)GaINAc-tetrasaccharide chains linked to serine or threonine residues 2 . The abundance of glycophorin A on the surface of RBCs is thought to create a negatively charged cell surface of complex glycans that allows for RBC circulation without sticking to other cells or blood vessel walls1 . Glycophorin A binds CD170 3 (Siglec 5, which mediates sialic-acid dependent binding to cells4), influenza virus, and EBA-175, the erythrocyte binding antigen of Plasmodium falciparum, the parasite responsible for malaria in Africa 2 . Babesia divergens, which like Plasmodium is a parasite in the Apicomplexa phylum, also uses glycophorins A and B to enter RBCs. Other pathogens that bind to glycophorin A on the surface of RBCs include: some strains of Escherichia coli, reoviruses (a group of viruses that includes rotavirus), encephalomyocarditis virus, and hepatitis A 2 . Glycophorin A also interacts with influenza virus but because influenza virus cannot replicate in anucleated RBCs glycophorin A might be acting as a decoy receptor to divert infection away from other tissues. JC159 was generated using splenic hairy cell leukemia cells as immunogen and reacts with a formalin-fixed, paraffin-embedded resistant epitope, likely between amino acids 27 and 40, on the extracellular domain5. Antigen Distribution Glycophorin A (CD235a) is expressed on hematopoietic stem cells,
erythroid cells, and erythrocytes. Erythroleukemia can express glycophorin A on neoplastic
erythroblasts. NCBI Gene Bank ID UniProt.org Research Area Cell Adhesion . Immunology . Infectious Disease . Parasite . Viral References & Citations1 Hollox EJ, Louzada S. 75(3):201-206. 2023. 2 Jaskiewicz E, Jodłowska M, Kaczmarek R, et al. Parasit Vectors. 12(1):317. 2019. 3 https://apps.vetmed.wsu.edu/tkp/report.aspx?ID=311 4 https://www.uniprot.org/uniprotkb/O15389/entry 5 Erber WN, McLachlan J, Cordell JL, et al. Hematology Reviews and Communications. 5(2):113-120. 1991. 6 Tommila M, Stark C, Jokilammi A, et al. J Mol Cell Biol. 3(3):190-196. 2011. 7 Sadahira Y, Kanzaki A, Wada H, et al. J Clin Pathol. 52(12):919-921. 1999. 8 Sutherland DR, Keeney M, Illingworth A. Cytometry B Clin Cytom. 82(4):195-208. 2012. Technical ProtocolsCertificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.