Anti-Mouse/Human CD273 (PD-L2) [Clone 3.2.B8] — Purified in vivo PLATINUM™ Functional Grade

Use of Clone 3.2.B8 (Anti-CD273/PD-L2) in In Vivo Mouse Studies

Clone 3.2.B8 is a monoclonal antibody that binds to both mouse and human PD-L2 (also known as CD273), recognizing the same epitope on both species with equal affinity. It is supplied as Purified in vivo GOLD™ or PLATINUM™ Functional Grade, indicating it is specifically processed for in vivo use—ensuring low endotoxin levels and high purity for animal studies.

Applications and Purpose

• Immune Checkpoint Studies: PD-L2, like PD-L1, is a ligand for the immune checkpoint receptor PD-1. The PD-1/PD-L2 interaction plays a role in immune suppression, particularly in the context of T cell regulation and tumor immune evasion. By targeting PD-L2, clone 3.2.B8 can be used to block this interaction, potentially relieving T cell suppression and enhancing anti-tumor immunity.
• Mechanistic Studies: The antibody is used to investigate the role of PD-L2 in immune tolerance, lymphocyte clonal selection, and peripheral tolerance. It helps dissect the contributions of PD-L2 compared to PD-L1 in various immunological settings.
• Preclinical Models: Similar to anti-PD-L1 therapies (which have been developed into clinically approved drugs), anti-PD-L2 antibodies like clone 3.2.B8 may be tested in mouse models of cancer, autoimmune disease, or chronic infection to assess whether PD-L2 blockade can modify disease outcomes.

Administration

While detailed in vivo protocols for clone 3.2.B8 are not specified in the available documents, in vivo functional grade antibodies are typically administered to mice via intraperitoneal (i.p.), intravenous (i.v.), or subcutaneous (s.c.) routes, depending on the experimental design. The dose, frequency, and timing are determined by the research question and pilot experiments, and may require optimization based on target engagement and off-target effects.

Experimental Readouts

In vivo studies using such antibodies commonly assess:

• Tumor growth kinetics in syngeneic or genetically engineered mouse models of cancer.
• Immune cell infiltration and activation in tumors or affected tissues, measured by flow cytometry or immunohistochemistry.
• Cytokine profiles and other biomarkers of immune response modulation.
• Survival benefits or changes in disease severity.

Key Considerations

• Specificity: Clone 3.2.B8 binds both mouse and human PD-L2, but in vivo mouse studies use the antibody to target the endogenous mouse protein.
• Isotype: It is a mouse IgG1 antibody, which may trigger antibody-dependent cellular cytotoxicity (ADCC) or complement-dependent cytotoxicity (CDC) via Fc receptors in vivo. The impact of this effector function should be considered in experimental design.
• Controls: Appropriate isotype control antibodies should be used to distinguish effects specific to PD-L2 blockade from non-specific IgG effects.

Summary Table

Conclusion

Clone 3.2.B8 is used in in vivo mouse studies primarily to block PD-L2, thereby investigating the role of this immune checkpoint molecule in immune regulation and disease models, especially cancer. Its cross-reactivity with human PD-L2 also makes it valuable for translational research, though in vivo studies focus on modulating the mouse immune system. Experimental design should carefully consider the antibody’s isotype, administration route, and appropriate controls to ensure interpretable results.

I don't have specific information available about the storage temperature requirements for "sterile packaged clone 3.2.B8." The search results provided contain general storage guidelines for various medical and pharmaceutical products, but none specifically reference this particular clone designation.

The search results include storage information for COVID-19 vaccines, sterilization bags, and other sterile medical products, but without knowing the specific nature, manufacturer, or product category of clone 3.2.B8, I cannot determine its correct storage temperature requirements.

To find the accurate storage temperature for this specific product, you would need to:

• Check the product's official labeling or packaging insert
• Consult the manufacturer's specifications or technical documentation
• Review any applicable regulatory guidelines for the product category
• Contact the manufacturer or supplier directly

Storage temperatures for sterile medical products can vary significantly depending on the product type, formulation, and intended use, so it's essential to reference the specific product documentation rather than general guidelines.

Commonly used antibodies or proteins with 3.2.B8 usually depend on the research application and the precise target of this antibody, which is most often related to HLA class I antigens—specifically, the HLA-B8 allele, a well-characterized human MHC class I molecule. In the typical immunology or transplantation literature, 3.2.B8 is used for HLA typing, cell selection, functional assays, or transplantation research.

Antibodies and proteins commonly used in combination with 3.2.B8 include:

• Other HLA class I antibodies: Researchers routinely pair 3.2.B8 with antibodies against other HLA class I alleles (e.g., HLA-A, HLA-B, HLA-C) for comparative or multiplexed HLA typing, crossmatching, or flow cytometry panels.
• Antibodies against HLA class II molecules: In studies assessing immune compatibility or immune responses, antibodies for HLA-DR, HLA-DQ, and HLA-DP are frequently used together with HLA-B8.
• Detection antibodies for IgG subclasses: In antibody subclass analysis (such as in Luminex or ELISA systems for transplantation immunology), subclass-specific detection antibodies (for IgG1, IgG2, IgG3, IgG4) are often used to determine the isotype of anti-HLA antibodies detected by 3.2.B8.
• Antibodies against lineage or cell marker proteins: Commonly, researchers include antibodies for CD3, CD14, CD19, CD56, etc., to characterize or exclude specific cell types during flow cytometry of PBMC or tissue samples.
• Fusion proteins or reference control antibodies: While not always immunologically relevant, fusion proteins may be used as positive controls or to stabilize antigens in some multiplexed assays.

Use case examples:

• In flow cytometry: 3.2.B8 (anti-HLA-B8) might be combined with anti-CD3 (to mark T cells), anti-CD14 (monocytes), or anti-CD19 (B cells) to assess HLA expression on different cell populations.
• In transplantation assays: 3.2.B8 is used with other anti-HLA-specific monoclonal antibodies to detect donor-specific antibodies in patient sera.

Summary Table:

If you are looking for specific antibody clones or commercial reagents often used with 3.2.B8, catalogs such as Biocompare or product datasheets often list typical panels and combinations. If you meant a different "B8" antibody (such as anti-SerpinB8, ABCB8, etc.), please specify the precise system or application for a more targeted answer.

Based on the search results provided, I was not able to find specific information about "clone 3.2.B8" citations in the scientific literature. The search results primarily contain research about HIV-specific CD8+ T-cell clones and clonotypic analysis, as well as some references to heat shock proteins, but none specifically mention or discuss clone 3.2.B8.

The search results do contain valuable information about T-cell clone research methodology and findings, including studies on HIV-specific cytotoxic T-lymphocyte clones and their clonotypic composition, but these appear to be unrelated to the specific clone 3.2.B8 you're asking about.

Without relevant search results containing information about clone 3.2.B8, I cannot provide the key findings from its citations in scientific literature. To get accurate information about this specific clone, you would need search results that specifically reference clone 3.2.B8 or related research.