Anti-Human TACE Ectodomain (Clone 111623)
Anti-Human TACE Ectodomain (Clone 111623)
Product No.: T461
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Clone 111623 Target TACE Ectodomain Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Tumor Necrosis Factor-α Converting Enzyme, ADAM17, CD156b, MGC71942, cSVP Isotype IgG1 Applications IP , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen T. ni Cell-Derived Recombinant Human TACE (Accession # P78536) Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831961 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human TACE this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human TACE is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? Immunoprecipitation: Suitable for use at concentration of 25 µg/mL. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Mouse Anti-Human TNF-α Coverting Enzyme (TACE) (Clone 111623) recognizes an epitope on Human TACE. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background TACE (tumor necrosis factor-α-converting enzyme) is a pleiotropic metalloprotease also known as ADAM17 that is found to be constitutively expressed by intestinal epithelial cells.1 TACE is a major sheddase of TNF-α and its receptors, essential for the generation of soluble, mature molecules.2 TACE as a promising target of epidermal growth factor receptor (EGFR) axis inhibition in colorectal cancer (CRC)3 and a principal target for the treatment of TNF-dependent pathologies.4 PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Masson, D. et al. (2009) Int J Mol Med. 23: 41 2. Chodynicka, B. et al. (2008) J European Acad Dermatol & Venereol. 22: 712 3. Coffey, RJ. et al. (2008) Clin Cancer Res. 14: 1182 4. Blobel, CP. et al. (2007) J Immunol. 179: 2689 Technical Protocols  Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.