Anti-Human TACE Ectodomain (Clone 111633)

Product No.: T460


Clone 111633 Target TACE Ectodomain Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Tumor Necrosis Factor-α Converting Enzyme, ADAM17, CD156b, MGC71942, cSVP Isotype IgG1 Applications IP , WB


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Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen T. ni Cell-Derived Recombinant Human TACE (Accession # P78536) Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831960 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human TACE this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human TACE is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Flow Cytometry: Suitable for use at concentration of 0.25 µg/10⁶ cells. Additional Applications Reported In Literature ? Immunoprecipitation: This antibody has been used to immunoprecipitate rhTACE (ecto) from conditioned media of transfected insect cells. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Mouse Anti-Human TNF-α Coverting Enzyme (TACE) (Clone 111633) recognizes an epitope on Human TACE. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows less than 5% cross-reactivity with the ecto domain of recombinant human ADAM8, 9 and 15 as well as mouse ADAM10. Background TACE (tumor necrosis factor-α-converting enzyme) is a pleiotropic metalloprotease also known as ADAM17 that is found to be constitutively expressed by intestinal epithelial cells.¹ TACE is a major sheddase of TNF-α and its receptors, essential for the generation of soluble, mature molecules.² TACE as a promising target of epidermal growth factor receptor (EGFR) axis inhibition in colorectal cancer (CRC)³ and a principal target for the treatment of TNF-dependent pathologies.⁴ PubMed TACE Ectodomain NCBI Gene Bank ID 6868 UniProt.org Information on Uniprot.org References & Citations 1. Masson, D. et al. (2009) Int J Mol Med. 23: 41 2. Chodynicka, B. et al. (2008) J European Acad Dermatol & Venereol. 22: 712 3. Coffey, RJ. et al. (2008) Clin Cancer Res. 14: 1182 4. Blobel, CP. et al. (2007) J Immunol. 179: 2689 View product citations for antibody T460 on CiteAb Technical Protocols Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

T. ni Cell-Derived Recombinant Human TACE (Accession # P78536)

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2831960

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Human TACE this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human TACE is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Flow Cytometry: Suitable for use at concentration of 0.25 µg/10⁶ cells.

Additional Applications Reported In Literature ?

Immunoprecipitation: This antibody has been used to immunoprecipitate rhTACE (ecto) from conditioned media of transfected insect cells.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human TNF-α Coverting Enzyme (TACE) (Clone 111633) recognizes an epitope on Human TACE. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows less than 5% cross-reactivity with the ecto domain of recombinant human ADAM8, 9 and 15 as well as mouse ADAM10.

Background

TACE (tumor necrosis factor-α-converting enzyme) is a pleiotropic metalloprotease also known as ADAM17 that is found to be constitutively expressed by intestinal epithelial cells.¹ TACE is a major sheddase of TNF-α and its receptors, essential for the generation of soluble, mature molecules.² TACE as a promising target of epidermal growth factor receptor (EGFR) axis inhibition in colorectal cancer (CRC)³ and a principal target for the treatment of TNF-dependent pathologies.⁴

PubMed

TACE Ectodomain

NCBI Gene Bank ID

6868

UniProt.org

Information on Uniprot.org

References & Citations

1. Masson, D. et al. (2009) Int J Mol Med. 23: 41
2. Chodynicka, B. et al. (2008) J European Acad Dermatol & Venereol. 22: 712
3. Coffey, RJ. et al. (2008) Clin Cancer Res. 14: 1182
4. Blobel, CP. et al. (2007) J Immunol. 179: 2689

View product citations for antibody T460 on CiteAb

Certificate of Analysis

Request COA

Prod No.	Description
T461	Anti-Human TACE Ectodomain (Clone 111623)

Formats Available

Prod No.	Description
T460	Anti-Human TACE Ectodomain (Clone 111633)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

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Anti-Human TACE Ectodomain (Clone 111633)