Anti-Human Tie-2 (Clone 83711)

Pricing & Details

Product No.T433
Clone
83711
Protein
Tie-2
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Tyrosine Kinase With Immunoglobulin And Epidermal Growth Factor Homology Domain 2, VMCM1, CD202b, TEK
Isotype
Mouse
IgG1
Applications
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
T433-25 µg
25 µg
$150.00
In stock
Max:
Min: 1
Step: 1
T433-500 µg
500 µg
$525.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human Tie-2 (Accession # AAA61139)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human Tie-2 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human Tie-2 is 5.0 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T388.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human Tie-2 recognizes an epitope on Human Tie-2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Tyrosine kinase with immunoglobulin-like and EGF-like domains 2 (Tie-2) are novel endothelial cell tyrosine kinase receptors that are essential for vascular development and remodeling in the embryo.1 They are expressed almost exclusively in endothelial cells.2 Tie-2 is functional in pericytes and may play an important role in the progression of diabetic retinopathy, by regulating pericyte loss and influencing the activation state and recruitment of pericytes.3 Tie2 has been shown to be up-regulated in psoriasis and in breast cancer tissue and blocking Tie2 action inhibits tumor angiogenesis and tumor growth.

Antigen Details

Protein
PubMed

References & Citations

1. Vartikovski, L. et al. (1999) J Vasc Res. 36: 272
2. Peters, KG. et al. (2002) Mol Cell Biol. 22: 1704
3. Boulton, ME. et al. (2008) Invest Ophthalmol Vis Sci 49: 2163
General Western Blot Protocol

Formats Available

Prod No.
Description
T433
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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