Anti-Human TNF alpha (Adalimumab)

Pricing & Details

Product No.LT100
Clone
D2E7
Protein
TNF alpha
Formats AvailableView All
Product Type
Biosimilar Recombinant Human Monoclonal Antibody
Alternate Names
DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha
Isotype
Human IgG1κ
Applications
B
,
ELISA
,
FA
,
FC
,
IF
,
IHC
,
IP
,
N
Prod No.
Size
Price
Avail.
Qty
Add to cart
LT100-Custom
Custom
In stock
Max:
Min: 1
Step: 1
LT100-1.0 mg
1.0 mg
$175.00
In stock
Max:
Min: 1
Step: 1
LT100-5.0 mg
5.0 mg
$650.00
In stock
Max:
Min: 1
Step: 1
LT100-25 mg
25 mg
$2,450.00
In stock
Max:
Min: 1
Step: 1
LT100-50 mg
50 mg
$3,750.00
In stock
Max:
Min: 1
Step: 1
LT100-100 mg
100 mg
$5,100.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Human
Expression Host
HEK-293
FC Effector Activity
Active
Immunogen
Human TNF alpha
Product Concentration
≥ 2.0 mg/ml
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This biosimilar antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s recombinant biosimilar antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Regulatory Status
Research Use Only (RUO). Non-Therapeutic.
Country of Origin
USA
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for Adalimumab biosimilar antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Other Applications Reported In Literature ?
N
B
ELISA
FA
IF
IP
IHC
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
This non-therapeutic biosimilar antibody uses the same variable region sequence as the therapeutic antibody Adalimumab. Clone D2E7 binds to soluble TNF- α, but not to TNF- β (lymphotoxin). This product is for research use only.
Antigen Distribution
TNF-α is secreted by macrophages, monocytes, neutrophils, T cells, B cells, NK cells, LAK cells.
Background
Adalimumab is a research-grade monoclonal antibody that works by inactivating tumor necrosis factor-alpha (TNF-α). TNF-α is a 17.5 kD protein that mediates inflammation and immunity caused by the invasion of viruses, bacteria, and parasites by initiating a cascade of cytokines that increase vascular permeability, thus bringing macrophages and neutrophils to the site of infection. TNF-α secreted by the macrophage causes the blood to clot which provides containment of the infection. TNF-α inactivation has proven to be important in downregulating the inflammatory reactions associated with autoimmune diseases such as rheumatoid arthritis, psoriatic arthritis, ankylosing spondylitis, Crohn's disease, moderate to severe chronic psoriasis, and juvenile idiopathic arthritis. Adalimumab blocks the interaction with the p55 and p75 cell surface TNF receptors thus, neutralizing the biological function of TNF. Anti-Human TNF alpha (Adalimumab) utilizes the same variable regions from the therapeutic antibody Adalimumab making it ideal for research projects.

Antigen Details

Protein
PubMed
NCBI Gene Bank ID

References & Citations

1. Omidinia, E. et al. (2019) Protein Expr Purif. 155:59-65.
Elisa Sandwich Protocol
Flow Cytometry
IHC
Immunoprecipitation Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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