Anti-Human TRAIL R2

Product No.: T451


Clone 71903 Target TRAIL R2 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names TNF-Related Apoptosis-Inducing Ligand Receptor 2, TNFRSF10B, CD262, DR5, KILLER, TRICK2, TRICK2A, TRICK2B, TRICKB, ZTNFR9 Isotype IgG1 Applications ELISA Indirect , FA , WB


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Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen NS0-Derived Recombinant Human TRAIL R2 Extracellular Domain (Accession # CAG46696) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831951 Applications and Recommended Usage? Quality Tested by Leinco Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as PN:M114. The detection sensitivity of this indirect ELISA for Human TRAIL R2 is approximately 2 ng/well. Western Blotting: To detect Human TRAIL R2 this monoclonal antibody can be used at a concentration of 5 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Functional Assay: The biological activity of this human TRAIL R2 antibody was measured by its ability to induce apoptosis on Jurkat cells. The ED50 of this effect is typically 0.02 - 0.06 μg/mL under the assay conditions described in the figure legend. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Mouse Anti-Human TNF-Related Apoptosis-Inducing Ligand Receptor 2 (TRAIL R2) (Clone 71903) recognizes an epitope on Human TRAIL R2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Tumor necrosis factor-related apoptosis-inducing ligand Receptor 2 (TRAIL-R2) is a cell-surface receptor involved in tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced cell-death signaling.¹ The death ligand TRAIL bears high potential as a new anticancer agent, as binding to the death receptors TRAIL-R1 or TRAIL-R2 triggers apoptosis in most cancer cells.² TRAIL-R2 has been shown to be associated with a decrease in the survival rates of breast cancer patients.³ PubMed TRAIL R2 NCBI Gene Bank ID 8795 UniProt.org Information on Uniprot.org References & Citations 1. Yoo, NJ. et al. (2001) Oncogene 20: 399 2. Eberle, J. et al. (2005) J Investigative Dermatol. 125: 1010 3. Rimm, D. et al. (2005) Clin Cancer Research 11 View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols FA Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

NS0-Derived Recombinant Human TRAIL R2 Extracellular Domain (Accession # CAG46696)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2831951

Applications and Recommended Usage?
Quality Tested by Leinco

Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as PN:M114. The detection sensitivity of this indirect ELISA for Human TRAIL R2 is approximately 2 ng/well.
Western Blotting: To detect Human TRAIL R2 this monoclonal antibody can be used at a concentration of 5 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Functional Assay: The biological activity of this human TRAIL R2 antibody was measured by its ability to induce apoptosis on Jurkat cells. The ED50 of this effect is typically 0.02 - 0.06 μg/mL under the assay conditions described in the figure legend.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human TNF-Related Apoptosis-Inducing Ligand Receptor 2 (TRAIL R2) (Clone 71903) recognizes an epitope on Human TRAIL R2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

Tumor necrosis factor-related apoptosis-inducing ligand Receptor 2 (TRAIL-R2) is a cell-surface receptor involved in tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced cell-death signaling.¹ The death ligand TRAIL bears high potential as a new anticancer agent, as binding to the death receptors TRAIL-R1 or TRAIL-R2 triggers apoptosis in most cancer cells.² TRAIL-R2 has been shown to be associated with a decrease in the survival rates of breast cancer patients.³

PubMed

TRAIL R2

NCBI Gene Bank ID

8795

UniProt.org

Information on Uniprot.org

References & Citations

1. Yoo, NJ. et al. (2001) Oncogene 20: 399
2. Eberle, J. et al. (2005) J Investigative Dermatol. 125: 1010
3. Rimm, D. et al. (2005) Clin Cancer Research 11

View product citations for antibody PRODUCT_CODE on CiteAb

Certificate of Analysis

Request COA

Prod No.	Description
T451	Anti-Human TRAIL R2
C2424	Anti-Mouse CD262 (TRAIL R2) – Purified Functional Grade
C2428	Anti-Mouse CD262 (TRAIL R2) – PE
T474	Anti-Mouse TRAIL R2
T406	Recombinant Mouse TRAIL R2
T501	Anti-Mouse TRAIL R2 – Biotin
T497	Anti-Human TRAIL R2 – Biotin
C2426	Anti-Mouse CD262 (TRAIL R2) – Biotin

Formats Available

Prod No.	Description
T451	Anti-Human TRAIL R2

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Anti-Human TRAIL R2