Anti-Human TRAIL R4 (Clone 104918)
Anti-Human TRAIL R4 (Clone 104918)
Product No.: T452
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Clone 104918 Target TRAIL R4 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names TNFRSF10D, Decoy Receptor 2 (DCR2), CD264, TRUNDD, TR4, TNF-Related Apoptosis-Inducing Ligand Receptor 4 Isotype IgG1 Applications ELISA Cap , FC , N , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Purified Recombinant Human TRAIL R4 (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831952 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 4 µg/ml. A suitable detection antibody is PN:T498 at a concentration of approximately 400 ng/ml. A suggested standard for this assay is PN:T403. Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing TRAIL R4. A suggested method would be to stain cells expressing TRAIL R4 with 0.25 µg per 1 - 2.5 x 106 cells in a total staining volume of ≤200 µl followed by PN:M1189. Western Blotting: To detect Human TRAIL R4 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human TRAIL R4 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T403. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Human TRAIL R4 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human TRAIL R4 (at 10 ng/ml) is 0.04 – 0.2 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Human TNF-Related Apoptosis-Inducing Ligand Receptor 4 (TRAIL R4) (Clone 104918) recognizes an epitope on Human TRAIL R4. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background TNF-related apoptosis-inducing ligand receptor 4 (TRAIL R4) is a decoy receptor that negatively regulate TRAIL-induced cytotoxicity by competing for ligand binding with TRAIL-R1 and TRAIL-R2.1 TRAIL R4 is expressed mainly on CD8+ and NK cells and induces apoptosis in many tumour cells, but not in normal cells. TRAIL-R4 contains partially truncated death domain and therefore is unable to induce apoptosis and serves as a negative regulator of apoptotic signaling by impairment death-inducing signaling complex (DISC) processing.1,2 Antigen DetailsPubMed References & Citations1. Chan, FK. et al. (2005) Proc Natl Acad Sci USA 102: 18099 2. Sanlioglu, S. et al. (2005) BMC Cancer 5: 54 Technical Protocols  N  |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
