Anti-Human TRAIL R4
Anti-Human TRAIL R4
Product No.: T472
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Target TRAIL R4 Product Type Polyclonal Antibody Alternate Names TNF-Related Apoptosis-Inducing Ligand Receptor 4, TNFRSF10D, Decoy Receptor 2 (DCR2), CD264, TRUNDD, TR4 Applications CyTOF® , ELISA Indirect , FC , IHC FFPE , N , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Goat Immunogen NS0-Derived Recombinant Human TRAIL R4 Extracellular Domain Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831972 Applications and Recommended Usage? Quality Tested by Leinco Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as PN:G505. The detection sensitivity of this indirect ELISA for Human TRAIL R4 is approximately 0.6 ng/well. Western Blotting: To detect Human TRAIL R4 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human TRAIL R4 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Flow Cytometry: Suitable for use at concentration of 2.5 µg/106 cells. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 10 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Neutralization: This antibody is useful for neutralization of Human TRAIL R4 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human TRAIL R4 (at 10 ng/ml) is 0.003 - 0.009 µg/ml. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Goat Anti-Human TNF-Related Apoptosis-Inducing Ligand Receptor 4 (TRAIL R4) recognizes Human TRAIL R4. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background TNF-related apoptosis-inducing ligand receptor 4 (TRAIL R4) is a decoy receptor that negatively regulate TRAIL-induced cytotoxicity by competing for ligand binding with TRAIL-R1 and TRAIL-R2.1 TRAIL R4 is expressed mainly on CD8+ and NK cells and induces apoptosis in many tumour cells, but not in normal cells. TRAIL-R4 contains partially truncated death domain and therefore is unable to induce apoptosis and serves as a negative regulator of apoptotic signaling by impairment death-inducing signaling complex (DISC) processing.1,2 Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org References & Citations1. Chan, FK. et al. (2005) Proc Natl Acad Sci USA 102:18099 2. Sanlioglu, S. et al. (2005) BMC Cancer 5:54 Technical ProtocolsCyTOF®   IHC FFPE N  |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
