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![Id3 antibody [2B11] detects Id3 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Id3 protein stained by Id3 antibody [2B11] (56209) diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 um.](https://www.leinco.com/wp-content/uploads/2025/01/qed-bioscience-anti-id3-antibody-56209-1.jpg)
Id3 antibody [2B11] detects Id3 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Id3 protein stained by Id3 antibody [2B11] (56209) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Id3 antibody [2B11] (56209) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](https://www.leinco.com/wp-content/uploads/2025/01/qed-bioscience-anti-id3-antibody-56209-2.jpg)
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Id3 antibody [2B11] (56209) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. - -
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Antibody DetailsProduct DetailsReactive Species Human ⋅ Mouse Host Species Mouse Immunogen Mouse Id3 gst-fusion protein (aa 1-119) expressed in E. coli. Product Concentration Lot Specific Formulation 10 mM PBS, pH 7.4. State of Matter Liquid Product Preparation Purified by Protein A affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles. Regulatory Status Research Use Only Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting and Immuno- precipitation: use at 1-5 ug/mL.
Positive controls: Id3 transfected Sol8 muscles cells and recombinant fusion protein. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This antibody recognizes human and mouse Id3. Background Id3 is a 14 kD protein and a member of the Id family of helix-loop-helix (HLH) proteins. Id proteins are believed to be dominant negative regulators of HLH involved in cell differentiation and implicated in the control of cell growth, cellular differentiation, apoptosis, and carcinogenesis. Function Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Involved in myogenesis by inhibiting skeletal muscle and cardiac myocyte differentiation and promoting muscle precursor cells proliferation. Inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. {PubMed:8437843}. NCBI Gene Bank ID UniProt.org Research Area Neuroscience References & CitationsTechnical ProtocolsICC IF   Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
