Anti-Imipramine [Clone IM12-379.2]

Imipramine conjugated to KLH.

This monoclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.2 - 7.4 with no carrier protein or preservatives added.

Liquid

Antibodies are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

This antibody is stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

Next Day 2-8°C

Mouse Monoclonal Antibody specific to Imipramine.

Anti-Imipramine Monoclonal Antibody (Clone IM12-379.2); Understanding the Target: What is Imipramine?

Imipramine is a prominent, first-generation tricyclic antidepressant (TCA) belonging to the dibenzazepine group. Historically one of the first major antidepressants developed, it is primarily prescribed for the treatment of major depressive disorders and childhood enuresis (inability to control urination). Imipramine functions by inhibiting the reuptake of neurotransmitters—specifically serotonin and norepinephrine—in the central nervous system, thereby elevating mood and alleviating depressive symptoms.

Due to its narrow therapeutic index and the potential for severe side effects or toxicity at high doses, precise monitoring of imipramine levels in patient samples is highly critical for both patient safety and therapeutic efficacy.

The Role of Imipramine Antibodies in Toxicology and TDM

Because TCAs like imipramine are frequently involved in clinical overdose scenarios, rapid and accurate detection methods are essential. The Anti-Imipramine monoclonal antibody plays a vital role in developing these diagnostic solutions. It is widely utilized by researchers and diagnostic manufacturers in:

- Therapeutic Drug Monitoring (TDM): Ensuring that a patient's systemic drug levels remain within the strict therapeutic window to prevent toxicity or sub-therapeutic dosing.

- Toxicology Screening: Facilitating the rapid detection of TCA levels in urine, serum, or plasma samples for emergency room diagnostics or forensic toxicology.

- Pharmacokinetic Research: Assisting pharmaceutical developers in tracing the metabolism and physiological distribution of imipramine and its active metabolites in pre-clinical studies.

Antibody Specifications: Clone IM12-379.2

Developed initially by QED Bioscience and available through Leinco Technologies, Clone IM12-379.2 is a highly specific mouse monoclonal antibody (Isotype: Mouse IgG2b). The antibody was developed using an immunogen consisting of imipramine conjugated to Keyhole Limpet Hemocyanin (KLH), ensuring a robust and specific immune response. This clone is rigorously quality-tested and optimized for Enzyme-Linked Immunosorbent Assays (ELISA). It demonstrates reliable performance in indirect ELISA formats, particularly when screened against imipramine conjugated to Bovine Serum Albumin (BSA) on the solid phase.

1.) O'Callaghan AM, Phillips OM, Williams DC. Antisera raised against the drug imipramine. J Neurochem. 1987 Oct;49(4):1091-5. doi: 10.1111/j.1471-4159.1987.tb09998.x. PMID: 3625201.

2.) Adamczyk M, Fishpaugh J, Harrington C, Johnson D, Vanderbilt A. Immunoassay reagents for psychoactive drugs. II. The method for the development of antibodies specific to imipramine and desipramine. J Immunol Methods. 1993 Aug 9;163(2):187-97. doi: 10.1016/0022-1759(93)90122-n. PMID: 8354888.