Anti-KCNQ4 K+ Channel Antibody (11512)
Anti-KCNQ4 K+ Channel Antibody (11512)
Product No.: 11512
- -
- -
Clone S43-6 Target KCNQ4 K+ Channel Formats AvailableView All Product Type Monoclonal Alternate Names KQT-like 4, Potassium channel subunitα KvLQT4, Voltage-gated potassium channel subunit Kv7.4 Isotype Mouse IgG1 Applications IHC , IP , WB , AM , ICC/IF |
Data
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-KCNQ4 Monoclonal Antibody, Clone N43/6 (11512). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-KCNQ4 Monoclonal Antibody (11512) at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) KCNQ4 Antibody (D) Composite.
Immunohistochemistry analysis using Mouse Anti-KCNQ4 Monoclonal Antibody, Clone N43/6 (11512). Tissue: hippocampus. Species: Human. Fixation: Bouin’s Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-KCNQ4 Monoclonal Antibody (11512) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
Immunohistochemistry analysis using Mouse Anti-KCNQ4 Monoclonal Antibody, Clone N43/6 (11512). Tissue: frozen brain section. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-KCNQ4 Monoclonal Antibody (11512) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.
Western Blot analysis of Rat tissue lysate showing detection of KCNQ4 protein using Mouse Anti-KCNQ4 Monoclonal Antibody, Clone N43/6 (11512). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KCNQ4 Monoclonal Antibody (11512) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. - -
- -
Antibody DetailsProduct DetailsReactivity Species Human/Mouse/Rat Host Species Mouse Immunogen Fusion protein aa 2-77 of human KCNQ4 (K+7.4, KvLQT4, accession number P56696). Product Concentration 1.0 mg/ml Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C. Avoid repeated freeze-thaw cycles. Regulatory Status For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1-10ug/mL. A band of ~77kDa is detected.Immunohistochemistry and
Immunocytochemistry: use at 0.1-1ug/mL. Immunofluorescence: use at 1-10ug/mL These are recommended concentrations; Enduser should determine optimal concentrations for their applications. Positive control: COS cell lysate transiently expressing KCNQ4. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This antibody recognizes human, mouse and rat KCNQ4. Background Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. The protein coded for by the KCNQ4 gene forms a potassium channel that is thought to play a critical role in the regulation of neuronal excitability, particularly in sensory cells of the cochlea. The current generated by this channel is inhibited by M1 muscarinic acetylcholine receptors and activated by retigabine, a novel anti-convulsant drug. Antigen DetailsFunction Probably important in the regulation of neuronal excitability. May underlie a potassium current involved in regulating the excitability of sensory cells of the cochlea. KCNQ4 channels are blocked by linopirdin, XE991 and bepridil, whereas clofilium is without significant effect. Muscarinic agonist oxotremorine-M strongly suppress KCNQ4 current in CHO cells in which cloned KCNQ4 channels were coexpressed with M1 muscarinic receptors. NCBI Gene Bank ID UniProt.org Research Area Ion Channels References & CitationsTechnical Protocols   AM ICC/IF |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
