Anti-KDEL Antibody (11076-200)
Anti-KDEL Antibody (11076-200)
Product No.: 11076-200
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Clone 10C3 Target KDEL Formats AvailableView All Product Type Monoclonal Isotype Mouse IgG2a Applications ELISA , ICC , WB |
Data
Reactivity with proteins corresponding to the apparent molecular mass of Grp78 and Grp94 in samples from mammals, birds, insects, and plants.
Lane 1: Grp78 (BiP) recombinant protein, Lane 2: RK-13, Lane 3: Mouse liver microsomes, Lane 4: Rat liver microsomes, Lane 5: Heat-shocked HeLa cell lysate.
Human spleen stained with #11076-200 at 10ug/ml.
Staining of ER in mouse C2C12 myoblasts transfected with wild-type mouse ADAM12 with #11076-200 at 10ug/ml. - -
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Antibody DetailsProduct DetailsReactive Species All Host Species Mouse Immunogen Peptide corresponding to aa 649-654 of rat Grp78 conjugated to KLH. Product Concentration Lot Specific Formulation This monoclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.2 - 7.4, 50% glycerol and 0.09% sodium azide as a preservative. State of Matter Liquid Product Preparation Antibodies are purified by a multi-step process including the use of affinity chromatography. Storage and Handling This antibody may be stored as received at 2-8°C for up to one month. For longer term storage, appropriately aliquot in working volumes without diluting and store at -20°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: Use at 4ug/ml. Reactivity with proteins corresponding to the apparent molecular mass of Grp78 and Grp94 in samples from mammals, birds, insects, and plants.
Immunohistochemistry: Use at 5- 10ug/ml. These are recommended concentrations. End users should determine optimal concentrations for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Mouse Monoclonal Antibody specific to KDEL Antibody Background The tetrapeptide KDEL, located at the carboxy-terminal sequences of luminal proteins, is a retrieval motif essential for the precise sorting of these proteins along the secretory pathway. KDEL proteins perform essential functions in the endoplasmic reticulum (ER) related to protein folding as well as assembly. The localization of chaperones and other soluble proteins to the ER is achieved by their continuous retrieval from post-ER compartments by the KDEL receptor which is a membrane protein localized in the Golgi apparatus. Research Area Neuroscience References & CitationsTechnical Protocols ICC  Certificate of Analysis |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
