Anti-Mouse KDEL Receptor [Clone KR-10]
Anti-Mouse KDEL Receptor [Clone KR-10]
Product No.: 11078
Data
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-KDEL Receptor Monoclonal Antibody, Clone KR-10 (11078). Tissue: NRK cells. Species: Rat. Primary Antibody: Mouse Anti-KDEL Receptor Monoclonal Antibody (11078) at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red). Counterstain: DAPI (blue) nuclear stain. KR-10 staining red; DAPI staining blue. Merged images. Courtesy of: Institute of Mol. and Cell Bio, Singapore.
Western Blot analysis of Rat tissue lysate showing detection of KDEL Receptor protein using Mouse Anti-KDEL Receptor Monoclonal Antibody, Clone KR-10 (11078). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KDEL Receptor Monoclonal Antibody (11078) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. - -
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Antibody DetailsProduct DetailsReactivity Species Bovine ⋅ Canine ⋅ Chicken ⋅ Drosophila ⋅ Hamster ⋅ Human ⋅ Monkey ⋅ Mouse ⋅ Ovine ⋅ Porcine ⋅ Rabbit ⋅ Rat ⋅ Xenopus Host Species Mouse Immunogen Peptide corresponding to aa 192-212 of bovine KDEL receptor Product Concentration 1.0 mg/ml Formulation PBS, pH 7.2, 50% glycerol, 0.09% sodium azide. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles. Regulatory Status For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Western blot: use at 1ug/ml. A band of approx. 25kDA is detected. Detection of KDEL receptor in rat tissue lysate with #11078 at 1ug/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Background KDEL receptor, an integral membrane protein, facilitates the retrieval of soluble endoplasmic
reticulum (ER) luminal proteins bearing the four amino acid retention motif KDEL from the Golgi
apparatus. The sequence of KDEL receptor is very similar to that of the yeast protein Erd2p, a
receptor for proteins containing the tetrapeptide HDEL, the yeast ER retention signal. KDEL
receptors interact with GAP (GTPase-activating protein) of ARF1, a small GTP-binding protein
involved in COPI-dependent vesicle transport. KEDL receptors may recruit cytosolic ARF1 to
membranes and regulate vesicle budding. Antigen DetailsFunction Receptor for the C-terminal sequence motif K-D-E-L that is present on endoplasmic reticulum resident proteins and that mediates their recycling from the Golgi back to the endoplasmic reticulum. {UniProtKB:P24390}. NCBI Gene Bank ID UniProt.org Research Area Neuroscience References & CitationsTechnical ProtocolsICC IF    |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
