Data
Immunoblotting: Detection of KT3-tagged fusion protein in 200, 100, and 50ng of E. coli cell lysate with 18854 diluted 1:5,000. - -
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Antibody DetailsProduct DetailsHost Species Goat Immunogen KT3 (KPPTPPPEPET) conjugated to KLH. Product Concentration 1.0 mg/ml Formulation PBS, pH 7.2, 0.09% sodium azide. State of Matter Liquid Product Preparation Purified by peptide affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at 2-8°C. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at a dilution of 1:1,000- 1:20,000
Immunocytochemistry: use at a dilution of 1:100-1:400. Immunoprecipitation: use 1-4ug/mg lysate ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100-1:500. These are recommended dilutions. End users should determine optimal dilutions for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Goat Polyclonal Antibody specific to KT3. Background The KT3 epitope tag is derived from SV40 large T antigen and corresponds to amino acids KPPTPPPEPET. It is frequently used as an epitope tag in expression vectors. Antigen DetailsResearch Area Epitope Tag References & CitationsTechnical Protocols ICC   |
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