Anti-Monkeypox Virus (Clone: MPXV-26)

Pricing & Details

Product No.LT553
Clone
MPXV-26
Protein
Monkeypox
Formats AvailableView All
Product Type
Recombinant Monoclonal Antibody
Alternate Names
MPXV
Isotype
Human IgG1λ
Applications
ELISA
,
N
Prod No.
Size
Price
Avail.
Qty
Add to cart
LT553-250 µg
250 µg
$390.00
Pre-order
Max:
Min: 1
Step: 1
LT553-1.0 mg
1.0 mg
$1,400.00
Pre-order
Max:
Min: 1
Step: 1
LT553-5 mg
5 mg
$5,250.00
Pre-order
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Monkeypox
Virus
Expression Host
HEK-293
Immunogen
MPXV-26 was generated from peripheral blood mononuclear cells obtained from donors who had recovered from naturally-occurring MPXV infection5.
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
Formulation
This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one year. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≥ -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Standard Overnight on Blue Ice.
Other Applications Reported In Literature ?
ELISA
N
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
MPXV-26 is reactive against L1 and MV surface protein. Complementary screening approaches were used to identify orthopoxvirus-specific mAbs to MPXV, cowpox virus (CPXV), variola virus (VARV), and vaccinia virus (VACV). MPXV-26 is reactive to VACV antigen, CPXV lysate, VARV antigen and lysate, but not MPXV lysate. MPXV-26 binds to VACV and VARV purified antigens and CPXV virus-infected cell lysate but not VACV and MPXV cell lysates.

MPXV-26 neutralizes VACV MV and MV plus complement (MV+C’), CPXV MV and MV+C’, and weakly neutralizes MPXV MV and MV+C’.

When MPXV-26 is mixed with other mAbs, neutralization and cross-neutralization are more efficient than in individual assays5. These mAb mixes also provide protection against VACV in mice. While not completely protective on its own, MPXV-26 induces delayed morbidity and mortality in mice, and mixes that exclude MPXV-26 are less effective prophylactics.
Antigen Distribution
L1 is a mature virion surface protein.
Background
Monkeypox virus (MPXV) is a zoonotic member of the Orthopoxvirus genus in the Poxviridae family1. It is the next most pathogenic poxvirus after smallpox. Two genetic clades, West African and Central African (Congo Basin), have been characterized; the latter is capable of human-to-human transmission1,2. Monkeypox has gained clinical relevance due to the eradication of smallpox, which has created opportunities for increased prevalence and viral mutations that may affect virulence1, 2. An infection with one orthopoxvirus of any one species, or vaccinia virus vaccination, protects against infection by other orthopoxviruses3,4,5. MPXV is an enveloped virus with a linear, double-stranded DNA genome2 and a large, complex proteome of over 200 proteins6. During infection, the virus exists in two antigenically distinct forms: mature virions (MV) or enveloped virions (EV)6.

Antigen Details

Protein
PubMed
Research Area
Infectious Disease
.
Monkeypox
.
Viral

References & Citations

1. Sklenovská N, Van Ranst M. Front Public Health. 6:241. 2018.
2. Moore M, Zahra F. 2021 Oct 19. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2022 Jan–.
3. McConnell S, Herman YF, Mattson DE, et al. Am J Vet Res. 25:192-195. 1964.
4. Hammarlund E, Lewis MW, Carter SV, et al. Nat Med. 11(9):1005-1011. 2005.
5. Gilchuk I, Gilchuk P, Sapparapu G, et al. Cell. 167(3):684-694.e9. 2016.
6. Moss B. Immunol Rev.239:8–26. 2011.
Elisa Sandwich Protocol

Formats Available

Prod No.
Description
LT553
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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