Anti-Monkeypox Virus (Clone: MPXV-56)

Pricing & Details

Product No.LT554
Clone
MPXV-56
Protein
Monkeypox
Formats AvailableView All
Product Type
Recombinant Monoclonal Antibody
Alternate Names
MPXV
Isotype
Human IgG1
Applications
ELISA
,
N
Prod No.
Size
Price
Avail.
Qty
Add to cart
LT554-250 µg
250 µg
$390.00
Pre-order
Max:
Min: 1
Step: 1
LT554-1.0 mg
1.0 mg
$1,400.00
Pre-order
Max:
Min: 1
Step: 1
LT554-5 mg
5 mg
$5,250.00
Pre-order
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Monkeypox
Virus
Expression Host
HEK-293
Immunogen
MPXV-56 was generated from peripheral blood mononuclear cells obtained from donors who had either recovered from naturally-occurring MPXV infection or been immunized against vaccinia5.
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
Formulation
This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one year. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≥ -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Standard Overnight on Blue Ice.
Other Applications Reported In Literature ?
ELISA
N
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone MPXV-56 is reactive against A33, a surface antigen on the EV form of the virus, and is of the IgG2 isotype.

Complementary screening approaches were used to identify orthopoxvirus-specific mAbs to MPXV, cowpox virus (CPXV), variola virus (VARV), and vaccinia virus (VACV). MPXV-56 is reactive against VACV antigen and lysate, CPXV lysate, MPXV lysate, and VARV antigen and lysate. MPXV-56 binds to VACV and VARV purified antigen and weakly binds virus-infected cell lysates of VACV, CPXV, and MPXV. MPXV-56 neutralizes VACV EV plus complement (+C’) but not MV, MV+C’, or EV.

MPXV-56 also neutralizes CPXV EV+C’ but not EV and weakly neutralizes MPXV EV+C’ but not MV, MV+C’, or EV.
Antigen Distribution
A33 is a surface antigen on the enveloped virion.
Background
Monkeypox virus (MPXV) is a zoonotic member of the Orthopoxvirus genus in the Poxviridae family1. It is the next most pathogenic poxvirus after smallpox. Two genetic clades, West African and Central African (Congo Basin), have been characterized; the latter is capable of human-to-human transmission1,2. Monkeypox has gained clinical relevance due to the eradication of smallpox, which has created opportunities for increased prevalence and viral mutations that may affect virulence1, 2. Rodents are thought to be the natural reservoir, with transmission through contact with bodily fluids and feces. Case fatality rates are <1% and up to 11% for the West African and Central Basin clades, respectively1,2. An infection with one orthopoxvirus of any one species, or vaccinia virus vaccination, protects against infection by other orthopoxviruses3,4,5.

MPXV is an enveloped virus with a linear, double-stranded DNA genome2 and a large, complex proteome of over 200 proteins6. During infection, the virus exists in two antigenically distinct forms: mature virions (MV) or enveloped virions (EV)6.

Antigen Details

Protein
PubMed
Research Area
Infectious Disease
.
Monkeypox
.
Viral

References & Citations

1. Sklenovská N, Van Ranst M. Front Public Health. 6:241. 2018.
2. Moore M, Zahra F. 2021 Oct 19. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2022 Jan–.
3. McConnell S, Herman YF, Mattson DE, et al. Am J Vet Res. 25:192-195. 1964.
4. Hammarlund E, Lewis MW, Carter SV, et al. Nat Med. 11(9):1005-1011. 2005.
5. Gilchuk I, Gilchuk P, Sapparapu G, et al. Cell. 167(3):684-694.e9. 2016.
6. Moss B. Immunol Rev.239:8–26. 2011.
Elisa Sandwich Protocol

Formats Available

Prod No.
Description
LT554
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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