Anti-Mouse Axl (Clone 107332)

Anti-Mouse Axl (Clone 107332)

Product No.: A383

[product_table name="All Top" skus="A383"]

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Clone
107332
Target
Axl
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Ark, Tyro7, Ufo, JTK11
Isotype
IgG1
Applications
ELISA Cap
,
WB

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
Purified Recombinant Mouse Axl (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2 and 100 µg/ml. A suitable detection antibody is PN:A405 at a concentration of approximately 200 ng/ml. A suggested standard for this assay is PN:A351.
Western Blotting: To detect Mouse Axl this monoclonal antibody can be used at a concentration of 1 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse Axl is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:A351.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse Axl (Clone 107332) recognizes an epitope on Mouse Axl. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Axl is a member of receptor tyrosine kinases characterized by an extracellular domains (ECD) resembling cell adhesion molecules and unique sequences in the kinase domain. In addition, Axl is transforming in both fibroblast and hematopoietic cells, and appears to be involved in mesenchymal development.1 Axl regulates cell migration, phagocytosis and apoptosis.2

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Liu, ET. et al. (1995) J Mol Biol. 270: 551
2. Berk, BC. et al. (2006) Circ Res. 98: 1446
Elisa Sandwich Protocol
General Western Blot Protocol
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Formats Available

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Prod No.
Description
A383
Products are for research use only. Not for use in diagnostic or therapeutic procedures.