Anti-Mouse CD19 – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Anti-Mouse CD19 – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Product No.: C525

[product_table name="All Top" skus="C2117"]

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Clone
1D3
Target
CD19
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
B4, CVID3
Isotype
IgG2a κ
Applications
IHC FF
,
PhenoCycler®

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CODEX® Details

Validation Notes
Clone 1D3 has been validated for use on CODEX® using mouse spleen fresh frozen (FF) tissue.
Tissue Screened
Spleen
Tissue Preparation
FF (Fresh Frozen)

Antibody and Reporter Details

Reactivity Species
Mouse
Host Species
Rat
Concentration
≥ 5.0 mg/ml
Immunogen
K562 cells expressing the extracellular domain of mouse CD19
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using <i>in vitro</i> cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Applications and Recommended Usage?
Quality Tested by Leinco
CODEX® This CD19 (Clone 1D3) antibody is formulated to simplify the antibody preparation needed when performing a CODEX® barcode conjugate. The suggested concentration is 0.5 mg/ml.
RRID
AB_2892848
Country of Origin
USA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 1D3 recognizes an epitope on mouse CD19.
Antigen Distribution
CD19 is expressed in the majority of Pro-B cells to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19.
Background
CD19 is a 95 kD transmembrane glycoprotein and member of the Ig superfamily. The antigen serves as an adaptor protein; drawing cytoplasmic signaling proteins to the membrane. It works via the CD19/CD21 complex to decrease the threshold for B cell receptor signaling pathways. Because of its presence on all B cells, CD19 is a biomarker for B lymphocyte development, lymphoma diagnosis and can be utilized as a target for the immunotherapy of lymphoproliferative disorders. Emerging studies indicate that CD19 plays an active role in fueling the growth of these cancers, most notably by stabilizing the concentrations of the MYC oncoprotein, making CD19 an attractive therapeutic target with respect to its downstream signaling.

Antigen Details

Protein
Ligand/Receptor
CD21, CD81, Leu-13.
Function
Modulates B cell activation and differentiation.
PubMed
NCBI Gene Bank ID
IHC
PhenoCycler®
Products are for research use only. Not for use in diagnostic or therapeutic procedures.