Anti-Mouse CD19 – Purified In vivo GOLD™ Functional Grade

Pricing & Details

Product No.C2117
Clone
1D3
Protein
CD19
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
B4, CVID3
Isotype
Rat
IgG2a κ
Applications
B
,
CODEX®
,
Depletion
,
FC
,
IHC FF
,
IP
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C2117-1.0 mg
1.0 mg
$95.00
In stock
Max:
Min: 1
Step: 1
C2117-5.0 mg
5.0 mg
$298.00
In stock
Max:
Min: 1
Step: 1
C2117-25 mg
25 mg
$800.00
In stock
Max:
Min: 1
Step: 1
C2117-50 mg
50 mg
$1,325.00
In stock
Max:
Min: 1
Step: 1
C2117-100 mg
100 mg
$1,998.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
K562 cells expressing the extracellular domain of mouse CD19
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<1.0 EU/µg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this 1D3 antibody for staining cells in flow cytometry is ≤ 1 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
WB The suggested concentration for this 1D3 antibody for use in western blotting is 1-10 μg/ml.
Other Applications Reported In Literature ?
CODEX®
B
Depletion
IP
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 1D3 recognizes an epitope on mouse CD19.
Antigen Distribution
CD19 is expressed in the majority of Pro-B cells to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19.
Background
CD19 is a 95 kD transmembrane glycoprotein and member of the Ig superfamily. The antigen serves as an adaptor protein; drawing cytoplasmic signaling proteins to the membrane. It works via the CD19/CD21 complex to decrease the threshold for B cell receptor signaling pathways. Because of its presence on all B cells, CD19 is a biomarker for B lymphocyte development, lymphoma diagnosis and can be utilized as a target for the immunotherapy of lymphoproliferative disorders. Emerging studies indicate that CD19 plays an active role in fueling the growth of these cancers, most notably by stabilizing the concentrations of the MYC oncoprotein, making CD19 an attractive therapeutic target with respect to its downstream signaling.

Antigen Details

Protein
Ligand/Receptor
CD21, CD81, Leu-13.
Function
Modulates B cell activation and differentiation.
PubMed
NCBI Gene Bank ID
Research Area
Immunology
Flow Cytometry
IHC
Immunoprecipitation Protocol
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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