Anti-Mouse CD209b – Purified in vivo PLATINUM™ Functional Grade

Pricing & Details

Product No.C6859
Clone
22D1
Protein
CD209b
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
DC-SIGNR1
Isotype
Armenian Hamster
IgG1 κ
Applications
B
,
FC
,
IHC FF
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C6859-1 mg
1 mg
$225.00
In stock
Max:
Min: 1
Step: 1
C6859-5 mg
5 mg
$445.00
In stock
Max:
Min: 1
Step: 1
C6859-25 mg
25 mg
$1,285.00
In stock
Max:
Min: 1
Step: 1
C6859-50 mg
50 mg
$2,045.00
In stock
Max:
Min: 1
Step: 1
C6859-100 mg
100 mg
$2,800.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Armenian Hamster
Immunogen
Purified Recombinant Mouse CD209b (C-terminal peptide)
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 22D1 recognizes an epitope on mouse CD209b.
Antigen Distribution
CD209b is predominantly expressed on macrophages in the spleen marginal zone and lymph nodes medulla.
Background
CD209b has a molecular weight of 37 kD and is a single-pass type II membrane protein containing a C-type lectin domain. CD209b plays a role in innate immune response, mediating the recognition and uptake of pathogen products such as lipopolysaccharides, pneumococcal polysaccharides, and dextrans. CD209b is a mouse homolog of human CD209, also known as DC-SIGN. Both primates and mice have several paralogs of CD209 that, within the species, are more comparable to each other than to orthologs of other species. Although DC-SIGN expression is similar in function and dendritic expression in both mice and humans, they are not unique orthologs. Higher primates have at least three DC-SIGN genes (DC-SIGN, DC-SIGNL1 and DC-SIGNL2); although DC-SIGNL2 has not been detected in humans. Up to eight paralogs of DC-SIGN have been reported in some laboratory mouse strains, some of which are differentially expressed on different cell types. On macrophages, DC-SIGN activates phagocytosis via the recognition and binding of mannose type carbohydrates, a class of pathogen associated molecular patterns (PAMPs) frequently found on viruses, bacteria and fungi. On myeloid and pre-plasmacytoid dendritic cells, DC-SIGN initiates immune response via the recognition of pathogen haptens (which elicits the production of antibodies), in addition to the mediation of dendritic cell rolling interactions with blood endothelium and activation of CD4+ T cells. Furthermore, DC-SIGN functions as receptor for viruses such as HIV and Hepatitis C. DC-SIGN, in conjunction with other C-type lectins, also plays a role in the recognition of tumors via dendritic cells. Hence, the therapeutic potential for DC-SIGN as a target for dendritic cell based cancer vaccine.

Antigen Details

Protein
Ligand/Receptor
CD209B binds to microbial polysaccharides and is a receptor for ICAM-3.
Function
CD209B is a C-type lectin receptor, mediating the recognition of various bacteria and viruses during innate immue response.
PubMed
NCBI Gene Bank ID
Flow Cytometry
IHC
General Western Blot Protocol