Anti-Mouse CD252 – Biotin

Anti-Mouse CD252 – Biotin

Product No.: C2338

[product_table name="All Top" skus="C2338"]

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Clone
RM134L
Target
CD252
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
OX 40 Ligand, OX40L, CD134L, TNFSF4
Isotype
IgG2b
Applications
FC

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
Rat NRK-52E cell line transfected with mouse OX-40L gene
Product Concentration
0.5 mg/ml
Formulation
This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Product Preparation
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. The antibody was then biotinylated using Leinco's in-house protocol.
Storage and Handling
This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. Each lot of this antibody is quality control tested
Additional Applications Reported In Literature ?
Additional reported applications (for the relevant formats)
B:The RM134L antibody can block the costimulatory activity of OX40L.
IHC-FFPE
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse CD252 (OX 40 Ligand), Clone RM134L recognizes an epitope on Mouse CD252.
Background
CD252 is a 35 kD member of the TNF superfamily and is the ligand for OX-40, which is primarily found on activated T cells. The CD252/OX-40 pair contributes to B-cell and T-cell interaction during the immune response. Both proliferative and IL-2 production responses of activated T cells are enhanced via OX-40 antigen stimulation, while proliferation and Ig secretion by activated B cells is enhanced via CD252 stimulation. Hence, because both T cells and B cells are activated in cross-linking, the CD252/OX-40 pair is considered reciprocally costimulatory.
Antigen Distribution
Activated B cells, antigen presenting cells

Antigen Details

Ligand/Receptor
OX40 (CD134)
Function
B-cell/T-cell interaction, costimulation
NCBI Gene Bank ID
Research Area
Costimulatory Molecules
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Immunology
Flow Cytometry

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.