Anti-Mouse CD252 (OX40L) – Purified in vivo PLATINUM™ Functional Grade

Pricing & Details

Product No.C6336
Clone
RM134L
Protein
CD252
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
OX 40 Ligand, OX40L, CD134L, TNFSF4
Isotype
Rat
IgG2b κ
Applications
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C6336-1.0 mg
1.0 mg
$225.00
In stock
Max:
Min: 1
Step: 1
C6336-5.0 mg
5.0 mg
$445.00
In stock
Max:
Min: 1
Step: 1
C6336-25 mg
25 mg
$1,285.00
In stock
Max:
Min: 1
Step: 1
C6336-50 mg
50 mg
$2,045.00
In stock
Max:
Min: 1
Step: 1
C6336-100 mg
100 mg
$2,800.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
Rat NRK-52E cell line transfected with mouse OX-40L gene
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FCFor flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume.
Other Applications Reported In Literature ?
B:The RM134L antibody can block the costimulatory activity of OX40L.
IHC-FFPE:
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone RM134L recognizes an epitope on mouse CD252.
Antigen Distribution
CD252 is expressed on activated B cells and antigen presenting cells.
Background
CD252 is a 35 kD member of the TNF superfamily and is the ligand for OX-40, which is primarily found on activated T cells. The CD252/OX-40 pair contributes to B-cell and T-cell interaction during the immune response. Both proliferative and IL-2 production responses of activated T cells are enhanced via OX-40 antigen stimulation, while proliferation and Ig secretion by activated B cells is enhanced via CD252 stimulation. Hence, because both T cells and B cells are activated in cross-linking, the CD252/OX-40 pair is considered reciprocally costimulatory.

Antigen Details

Protein
Ligand/Receptor
OX40 (CD134)
Function
B-cell/T-cell interaction, costimulation
PubMed
NCBI Gene Bank ID
Research Area
Costimulatory Molecules
.
Immunology
General Western Blot Protocol

Formats Available