Antibody DetailsProduct DetailsReactivity Species Mouse Host Species Rat Immunogen Rat NRK-52E cell line transfected with mouse OX-40L gene Product Concentration ≥ 5.0 mg/ml Endotoxin Level ≤ 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2737465 Applications and Recommended Usage? Quality Tested by Leinco FC For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. Each lot of this antibody is quality control tested. Other Applications Reported In Literature ? Additional reported applications (for the relevant formats) include: Immunohistochemical Staining Western Blotting Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Clone RM134L recognizes an epitope on mouse CD252. Antigen Distribution CD252 is expressed on activated B cells and antigen presenting cells. Background CD252 is a 35 kD member of the TNF superfamily and is the ligand for OX-40, which is primarily found on activated T cells. The CD252/OX-40 pair contributes to B-cell and T-cell interaction during the immune response. Both proliferative and IL-2 production responses of activated T cells are enhanced via OX-40 antigen stimulation, while proliferation and Ig secretion by activated B cells is enhanced via CD252 stimulation. Hence, because both T cells and B cells are activated in cross-linking, the CD252/OX-40 pair is considered reciprocally costimulatory. Antigen DetailsProtein Ligand/Receptor OX40(CD134) Function B-cell/T-cell interaction, costimulation PubMed NCBI Gene Bank ID UniProt.org Research Area Costimulatory Molecules . Immunology Technical Protocols |
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