Anti-Mouse CD29 [Clone KMI6] – CF®594

B cell-supportive stromal cell line BMS2

This CF® 594 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Liquid

Research Use Only

2 – 8° C Wet Ice

Red Laser (590 nm)

Stromal cells are needed for the early steps of B lymphocyte differentiation and proliferation¹. CD29, also known as integrin beta 1², is a 110 kDa protein discovered due to its expression on bone marrow stromal cells, at cell surface areas that interact with undifferentiated lymphoid cells¹. Integrin beta 1 is a member of a family of alpha beta heterodimers that act as cell surface receptors for extracellular matrix proteins³, including collagen, fibronectin, fibrinogen, laminin, VCAM1, and vitronectin among others⁴. CD29 is also involved in sperm-egg fusion. Through these interactions, CD29 regulates and/or promotes cell adhesion, laminin matrix deposition, endothelial cell motility, angiogenesis, osteoblast compaction, the formation of mineralized bone nodules, and cytokinesis.

KMI6 was raised against the B cell-supportive stromal cell line BMS2¹. LOU/MN rats were immunized with BMS2 cells and subsequently spleen cells were fused with murine Sp2/0 for hybridoma generation. Antibody recognition is insensitive to the glycosylation state but is lost when the CD29 protein is reduced for Western blotting². As such, the epitope is likely conformation-dependent. KMI6 was used to identify CD29². KMI6 can enhance cell adhesion of fibronectin to CD4 ^-8^- thymocytes³.

CD29 is present on the plasma membranes of many stromal cells, bone

Alpha-1, alpha-2, alpha-3, alpha-4, alpha-5, alpha-6, alpha-7, alpha-8, alpha-9, alpha-10, alpha-11 or alpha-V, collagen, fibronectin, fibrinogen, laminin, VCAM1, and vitronectin among others

1. Jacobsen K, Miyake K, Kincade PW, et al. J Exp Med. 176(4):927-935. 1992.
2. Wu X, Miyake K, Medina KL, et al. Hybridoma. 13(5):409-416. 1994.
3. Wadsworth SA, Chang AC, Hong MJ, et al. J Immunol. 154(5):2125-2133. 1995.
4. https://www.uniprot.org/uniprotkb/P09055/entry