Anti-Mouse CD29 (Clone KMI6) – Biotin

B cell-supportive stromal cell line BMS2

This Biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Liquid

This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.

Research Use Only

2 – 8° C Wet Ice

KMI6 activity is directed against mouse CD29, also known as integrin beta 1.

Stromal cells are needed for the early steps of B lymphocyte differentiation and proliferation¹. CD29, also known as integrin beta 1², is a 110 kDa protein discovered due to its expression on bone marrow stromal cells, at cell surface areas that interact with undifferentiated lymphoid cells¹. Integrin beta 1 is a member of a family of alpha beta heterodimers that act as cell surface receptors for extracellular matrix proteins³, including collagen, fibronectin, fibrinogen, laminin, VCAM1, and vitronectin among others⁴. CD29 is also involved in sperm-egg fusion. Through these interactions, CD29 regulates and/or promotes cell adhesion, laminin matrix deposition, endothelial cell motility, angiogenesis, osteoblast compaction, the formation of mineralized bone nodules, and cytokinesis.

KMI6 was raised against the B cell-supportive stromal cell line BMS2¹. LOU/MN rats were immunized with BMS2 cells and subsequently spleen cells were fused with murine Sp2/0 for hybridoma generation. Antibody recognition is insensitive to the glycosylation state but is lost when the CD29 protein is reduced for Western blotting². As such, the epitope is likely conformation-dependent. KMI6 was used to identify CD29². KMI6 can enhance cell adhesion of fibronectin to CD4 ^-8^- thymocytes³.

CD29 is present on the plasma membranes of many stromal cells, bone marrow stromal cell line BMS2 in vitro, and a small number of cells in bone marrow cell suspensions from adult mice. CD29 is also expressed in the media layer of the arterial wall. CD29 is expressed in skeletal and cardiac muscles. CD29 is also constitutively expressed by some immature thymocytes. CD29 can be slightly induced on mature thymocytes and peripheral T cells by activation with Con A.

Alpha-1, alpha-2, alpha-3, alpha-4, alpha-5, alpha-6, alpha-7, alpha-8, alpha-9, alpha-10, alpha-11 or alpha-V, collagen, fibronectin, fibrinogen, laminin, VCAM1, and vitronectin among other

1. Jacobsen K, Miyake K, Kincade PW, et al. J Exp Med. 176(4):927-935. 1992.
2. Wu X, Miyake K, Medina KL, et al. Hybridoma. 13(5):409-416. 1994.
3. Wadsworth SA, Chang AC, Hong MJ, et al. J Immunol. 154(5):2125-2133. 1995.
4. https://www.uniprot.org/uniprotkb/P09055/entry