Anti-Mouse CD90 (Thy-1) (Clone HK2.1) – Purified in vivo GOLD™ Functional Grade

Clone HK2.1 is a rat monoclonal antibody that targets mouse CD90 (Thy-1) and is commonly used in vivo in mice for several key applications, primarily related to T cell biology and stem cell research.

Common in vivo applications of clone HK2.1 in mice include:

• Cell depletion: Used to remove CD90+ cell populations (mainly T cells), allowing researchers to study the effects of T cell loss or depletion in immunological and disease models.
• Functional blockade: Applied to inhibit CD90 (Thy-1) signaling or adhesion, thus blocking downstream cellular interactions or pathways.
• Disease modeling: Facilitates studies of fibrosis, cancer, and neuronal injury by manipulating or monitoring CD90+ cells in pathophysiological contexts.
• Flow cytometry (FACS): Provides a tool for the detection and analysis of CD90+ cells, including T cells, thymocytes, and stem cell subsets, both for in vivo tracking and ex vivo analysis.

Supporting context:

• Clone HK2.1 recognizes a non-polymorphic determinant on murine CD90, enabling its use across different mouse strains (Thy1.1 and Thy1.2).
• In functional studies, it can be used to analyze CD90’s role in cell adhesion, stem cell regulation, and neuronal processes.
• Applications also include use as a marker in stem cell biology (e.g., hematopoietic stem cells, neural progenitors).
• The antibody is formulated for in vivo use (ultra low endotoxin), supporting direct administration in living animals.

Summary of main in vivo research uses:

• T cell (or other CD90+ cell) depletion and functional studies
• Modeling immune or non-immune disease states
• Tracking, isolating, or depleting stem/progenitor cells

These diverse uses make clone HK2.1 a fundamental tool for dissecting the biology of CD90+ cell populations in mouse models.

HK2.1 is a monoclonal antibody that targets mouse CD90 (Thy-1), often used in research involving stem cells and cancer. While there is no specific literature detailing antibodies or proteins commonly used with HK2.1, I can provide some insights based on related research:

1. CD90 (Thy-1): This marker is often used in combination with other stem cell markers like CD34 for identifying and isolating specific cell populations.

2. CD45: This marker interacts with CD90 in signal transduction processes and is frequently used in studies involving immune cells.

3. HK2 (Hexokinase 2): Although not directly paired with HK2.1, Hexokinase 2 is a protein of interest in cancer research, particularly in metabolic studies and as a potential therapeutic target.

4. HK2 (Human Kallikrein 2): This serine protease is studied in the context of prostate cancer and may be paired with other markers or antibodies in research involving prostate-specific antigens.

In summary, while HK2.1 itself is primarily used for its specificity to CD90, research involving other markers like CD34, CD45, and proteins such as Hexokinase 2 or Human Kallikrein 2 might intersect with studies employing HK2.1, particularly in cancer or stem cell research contexts.

The key findings regarding clone HK2.1 in scientific literature are limited and mainly relate to its use as an anti-mouse CD90 (Thy-1) antibody for in vivo and in vitro immunological research, particularly in murine models. There is no evidence in the search results of clone HK2.1 being directly cited in high-impact publications for specific biological discoveries beyond its use as a reagent.

Key points regarding clone HK2.1:

• Clone HK2.1 is an antibody directed against mouse CD90 (Thy-1), a cell surface glycoprotein expressed on thymocytes, mature T cells, neurons, and other cell types in mice.
• It is functionally validated for in vivo and in vitro applications, primarily to identify, deplete, or track CD90+ cells in mouse studies.
• There are no citations in high-profile scientific literature highlighted in the search results that describe novel findings or specific discoveries made using clone HK2.1 as a central methodological tool. It is referenced mainly as a standard reagent for immunological assays or animal experiments.
• Alternative clones, such as M5/49.4.1, are sometimes compared or used interchangeably for similar purposes in immunological research.

Summary:
Clone HK2.1 is widely used as a technical antibody tool for mouse CD90 (Thy-1) research; however, its citations are predominantly in the context of experimental methods rather than as the basis for major biological findings or discoveries. If you are seeking specific landmark studies or applications enabled by clone HK2.1, the literature does not currently provide prominent examples.

If you were referring to a different target (such as HK2 or hK2), please clarify to enable a more targeted review.

The search results do not provide specific information on how dosing regimens of clone HK2.1 vary across different mouse models. Clone HK2.1 is an antibody used to recognize the non-polymorphic determinant on mouse CD90 (Thy1.1 and Thy1.2 alloantigens) and is involved in various biological processes, including cell adhesion and regulation of hematopoietic stem cells. For detailed dosing regimens, one would typically need to consult product-specific datasheets or research studies that use this antibody in different mouse models.

In general, dosing regimens for antibodies in mouse models can vary based on factors such as the target population, mouse strain, and experimental design. Doses can range significantly depending on the application and desired outcome, such as immune cell depletion or modulation of immune responses. However, specific dosing regimens for clone HK2.1 will depend on the specific research goals and model being used.