Anti-Mouse CTLA-4 (CD152) [Clone UC10-4F10-11] — Purified in vivo GOLD™ Functional Grade
Anti-Mouse CTLA-4 (CD152) [Clone UC10-4F10-11] — Purified in vivo GOLD™ Functional Grade
Product No.: C2860
Clone UC10-4F10-11 Target CTLA-4 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names CD152, Cytotoxic T Lymphocyte-Associated Antigen-4, Ly-56 Isotype Armenian Hamster IgG1 κ Applications FA , FC , in vivo , IP , WB |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Armenian Hamster Recommended Isotype Controls Recommended Dilution Buffer Immunogen Purified Recombinant Mouse CTLA4 Fc Fusion Protein Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2829616 Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone UC10-4F10-11 recognizes an epitope on mouse CTLA-4. Background CTLA-4 is a 33 kD member of the Ig superfamily similar to CD28 in amino acid sequence, structure, and genomic organization. CTLA-4 is a protein receptor that functions as an immune checkpoint and downregulates immune responses. It is involved in the development of protective immunity and thymocyte regulation, in addition to the induction and maintenance of immunological tolerance. CTLA-4 has therapeutic potential both as an agonist to reduce immune activity, and an antagonist to increase immune activity. Antigen Distribution CTLA-4 is expressed on activated T and B lymphocytes. Ligand/Receptor CD80 (B7-1), CD86 (B7-2) Function Negative regulator of T cell activation NCBI Gene Bank ID UniProt.org Research Area Immunology . Inhibitory Molecules Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. The clone UC10-4F10-11 is widely used in in vivo mouse studies as an Armenian hamster IgG monoclonal antibody that specifically targets mouse CTLA-4 (CD152) and serves as a potent neutralizing antibody for CTLA-4. In vivo, UC10-4F10-11:
According to manufacturers and published literature, this antibody is offered in low endotoxin preparations for in vivo applications, and recommended isotype controls are available to ensure specificity in animal studies. Researchers typically select the UC10-4F10-11 clone for its documented ability to neutralize CTLA-4 both in vitro and in vivo, thereby facilitating enhanced T cell immune responses in experimental mouse models. The correct storage temperature for the sterile packaged clone UC10-4F10-11 (anti-mouse CTLA-4 antibody) is 2–8°C (typically 4°C), undiluted, and protected from prolonged exposure to light. Do not freeze the antibody, as this can compromise its integrity and activity.
Always consult the lot-specific datasheet, as some suppliers may recommend small variations, but 2–8°C (refrigerated, not frozen) is the consensus for the sterile packaged antibody. Other commonly used antibodies or proteins with UC10-4F10-11 in the literature include additional anti-CTLA-4 antibody clones, isotype controls, and protein ligands relevant to T-cell co-stimulation and regulation. Key examples:
Experimental approaches frequently involve the simultaneous detection of CTLA-4 (CD152) or the use of Western blot or flow cytometry with UC10-4F10-11, supported by these controls and other clones for comparison of specificity, depletion efficacy, and cross-reactivity. In summary, commonly paired or compared reagents include:
These combinations enable precise study of T cell regulation, co-stimulation, and tumor immunology. Key Findings from Clone UC10-4F10-11 CitationsClone UC10-4F10-11 is an Armenian hamster IgG monoclonal antibody used to target mouse CTLA-4 (CD152), primarily in preclinical research. Below is a summary of the most significant scientific findings and applications associated with this clone, based on the available literature: Functional and Mechanistic Insights
Immunological Effects in Animal Models
Technical and Application Notes
Summary Table: Key Attributes of UC10-4F10-11
ConclusionClone UC10-4F10-11 is a well-characterized tool for studying CTLA-4 function in mice. Its key scientific findings include potent blockade of CTLA-4-mediated inhibition, induction of IL-10-producing regulatory T cells, skewing of immune responses toward a Th2 phenotype, and protective effects in models of autoimmunityhighlighting its utility for dissecting mechanisms of immune regulation and tolerance. References & CitationsTechnical ProtocolsCertificate of Analysis |
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