Anti-Mouse E-Cadherin (Clone 114420)

Product No.: E324


Clone 114420 Target Epithelial Cadherin Formats AvailableView All Product Type Monoclonal Antibody Alternate Names ECAD Isotype IgG2a Applications CyTOF® , FC , IHC FFPE , WB


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Antibody Details Product Details Reactive Species Mouse Host Species Mouse Immunogen Purified Recombinant Mouse E-Cadherin (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Small package size (25μg) is supplied as either lyophilized or as a 0.2 μm filtered solution in PBS. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles.* No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830084 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse E-Cadherin this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Mouse E-Cadherin is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:C1355. Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing E-Cadherin . A suggested method would be to stain cells expressing E-Cadherin with 0.25 µg/10⁶ cells in a total staining volume of 200 µl followed by PN:M1189. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 10 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity Mouse Anti-Mouse Epithelial Cadherin (E-Cadherin ) (Clone 114420) recognizes an epitope on Mouse E-Cadherin. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. PubMed Epithelial Cadherin NCBI Gene Bank ID 12550 UniProt.org Information on Uniprot.org References & Citations View product citations for antibody E324 on CiteAb Technical Protocols CyTOF® IHC FFPE Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Mouse

Host Species

Mouse

Immunogen

Purified Recombinant Mouse E-Cadherin (>98%)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. *Small package size (25μg) is supplied as either lyophilized or as a 0.2 μm filtered solution in PBS.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2830084

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Mouse E-Cadherin this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Mouse E-Cadherin is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:C1355.
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing E-Cadherin . A suggested method would be to stain cells expressing E-Cadherin with 0.25 µg/10⁶ cells in a total staining volume of 200 µl followed by PN:M1189.

Additional Applications Reported In Literature ?

IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 10 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Mouse Epithelial Cadherin (E-Cadherin ) (Clone 114420) recognizes an epitope on Mouse E-Cadherin. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

PubMed

Epithelial Cadherin

NCBI Gene Bank ID

12550

UniProt.org

Information on Uniprot.org