Antibody DetailsProduct DetailsReactivity Species Mouse Host Species Mouse Immunogen Purified Recombinant Mouse E-Cadherin (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830084 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse E-Cadherin this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Mouse E-Cadherin is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:C1355. Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing E-Cadherin . A suggested method would be to stain cells expressing E-Cadherin with 0.25 µg/106 cells in a total staining volume of 200 µl followed by PN:M1189. Other Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 10 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Mouse Epithelial Cadherin (E-Cadherin ) (Clone 114420) recognizes an epitope on Mouse E-Cadherin. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Antigen DetailsProtein PubMed NCBI Gene Bank ID UniProt.org Technical Protocols   |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.