Anti-Mouse EphA7
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Mouse Immunogen NS0 - Derived Recombinant Mouse EphA7 Extracellular Domain Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830037 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse EphA7 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Mouse EphA7 is 0.5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Mouse EphA7 recognizes Mouse EphA7. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background EPH receptor A7, also known as EPHA7 is a receptor tyrosine kinase of the Eph family.1 EPHA7 functions as a cell-cell contact-mediated signalling in dorsal limb patterning and/or axon guidance.2 EphA7 is expressed early in cortical development, becoming concentrated in anterior and posterior domains.3 EphA7 may have roles in the pathogenesis and development of gastric4 and colorectal5 carcinomas. Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org References & Citations1. Muir, EM. et al. (1999) Brain Res Mol Brain Res. 74: 225 2. Nieto, MA. et al. (1998) Development 125: 4195 3. Donoghue, MJ. et al. (2006) J Comp Neurol. 496: 627 4. Zhou, X. et al. (2007) Hum Pathol. 38: 1649 5. Sugimura, H. et al. (2005) Oncogene 24: 5637 Technical Protocols |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
