Anti-Mouse FOXP3 [MF-14] – Dylight® 550

This DyLight® 550 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

This DyLight® 550 conjugate is stable when stored at 2-8°C. Do not freeze.

Next Day 2-8°C

Yellow Laser (563 nm)

MF-14 activity is directed against mouse FOXP3.

FOXP3 is a transcription factor¹ required for development of CD4⁺CD25⁺ regulatory T cells (Treg) that does not affect CD4⁺CD25⁻ T cell proliferation². Treg cells expressing FOXP3 play a critical role in the maintenance of immune homeostasis and prevention of autoimmunity¹. FOXP3 promotes the generation of Tregs, represses cytokine expression in primary CD4⁺ T cells, and suppresses the transcriptional activities of NFAT and NF-κB³. FOXP3 also inhibits basal activation of PKC-θ, IκB kinase β (IKKβ), and TNF-α stimulation of NF-κB. Depletion of FOXP3⁺CD4⁺ Treg cells and/or loss of function mutations of FOXP3 results in severe autoimmunity, allergy, immunopathology as well as cancer immunity¹.

FOXP3 is predominantly expressed in CD25⁺CD4⁺ Tregs in the thymus and periphery^2,4, and abundance increases upon in vitro activation of CD4⁺CD25⁺ Tregs². Additionally, expression of FOXP3 in naïve T cells can convert these cells to a Treg phenotype⁴. FOXP3 is virtually undetectable in resting and activated CD4⁺CD25⁻ T cells².

FOXP3 represents a more specific marker for CD4⁺ Tregs than e.g., CD25, CD45RB, CTLA-4, and GITR which do not discriminate between Tregs and activated, effector, or memory T cells⁴.

FOXP3 is a unique marker of CD4⁺CD25⁺ regulatory T cells, distinguishing them from activated CD4⁺CD25⁻ T cells.