Anti-Mouse FOXP3 – APC

Product No.: F403

[product_table name="All Top" skus="F1193"]

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Clone
MF-14
Target
Foxp3
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2
Isotype
IgG1 κ
Applications
ELISA
,
FA
,
FC
,
ICFC
,
IF
,
WB

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Select Product Size
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Mouse
Product Concentration
0.2 mg/ml
Formulation
This Allophycocyanin (APC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This Allophycocyanin (APC) conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Excitation Laser
Red Laser (650 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
FC
Additional Applications Reported In Literature ?
IF,
ICFC
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
MF-14 activity is directed against mouse FOXP3.
Background
FOXP3 is a transcription factor1 required for development of CD4+CD25+ regulatory T cells (Treg) that does not affect CD4+CD25 T cell proliferation2. Treg cells expressing FOXP3 play a critical role in the maintenance of immune homeostasis and prevention of autoimmunity1. FOXP3 promotes the generation of Tregs, represses cytokine expression in primary CD4+ T cells, and suppresses the transcriptional activities of NFAT and NF-κB3. FOXP3 also inhibits basal activation of PKC-θ, IκB kinase β (IKKβ), and TNF-α stimulation of NF-κB. Depletion of FOXP3+CD4+ Treg cells and/or loss of function mutations of FOXP3 results in severe autoimmunity, allergy, immunopathology as well as cancer immunity1.

FOXP3 is predominantly expressed in CD25+CD4+ Tregs in the thymus and periphery2,4, and abundance increases upon in vitro activation of CD4+CD25+ Tregs2. Additionally, expression of FOXP3 in naïve T cells can convert these cells to a Treg phenotype4. FOXP3 is virtually undetectable in resting and activated CD4+CD25 T cells2.

FOXP3 represents a more specific marker for CD4+ Tregs than e.g., CD25, CD45RB, CTLA-4, and GITR which do not discriminate between Tregs and activated, effector, or memory T cells4.
Antigen Distribution
FOXP3 is a unique marker of CD4+CD25+ regulatory T cells, distinguishing them from activated CD4+CD25 T cells.

Antigen Details

PubMed
NCBI Gene Bank ID
Research Area
Cancer
.
Immuno-Oncology
.
Immunology
.
Transcription Factors
Indirect Elisa Protocol
FA
Flow Cytometry
ICFC
IF
General Western Blot Protocol

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.