Anti-Mouse Frizzled-4 – Biotin

Anti-Mouse Frizzled-4 – Biotin

Product No.: F1090

[product_table name="All Top" skus="F1090"]

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Target
Frizzled-4
Product Type
Polyclonal Antibody
Alternate Names
EVR1, FEVR, FZD4S, Fz-4, FzE4, GPCR, MGC34390, CD344
Applications
WB

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Goat
Immunogen
Purified Recombinant Mouse FZD4 (Accession # Q61088)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse FZD4 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Mouse FZD4 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is F1028.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Mouse Frizzled-4 (Fz4) recognizes Mouse Fz4. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Frizzled-4 (FZD4) is a Frizzled Receptor involved in mediating Wnt signaling that is widely expressed, particularly in heart, skeletal muscle, ovary, and fetal kidney. FZD4 functions as a ligand-receptor pair to control vascular development in the retina and inner ear.1 Fz4 expression may play a critical role in responses to Wnt signaling in the tumor microenvironment.2

Antigen Details

NCBI Gene Bank ID

References & Citations

1. Nathans, J. et al. (2007) J Biol Chem. 282: 4057
2. Holcombe, RF. et al. (2007) BMC Cell Biol. 8: 12
General Western Blot Protocol

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.