Anti-Mouse Frizzled-4

Anti-Mouse Frizzled-4

Product No.: F1058

[product_table name="All Top" skus="F1058"]

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Target
Frizzled-4
Product Type
Polyclonal Antibody
Alternate Names
EVR1, FEVR, FZD4S, Fz-4, FzE4, GPCR, MGC34390, CD344
Applications
IHC FFPE
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WB

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Goat
Immunogen
Purified Recombinant Mouse FZD4 (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse Frizzled-4 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse Frizzled-4 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:F1028.
Additional Applications Reported In Literature ?
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 15 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Mouse Frizzled-4 recognizes Mouse Frizzled-4. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Frizzled-4 (FZD4) is a Frizzled Receptor involved in mediating Wnt signaling that is widely expressed, particularly in heart, skeletal muscle, ovary, and fetal kidney. FZD4 functions as a ligand-receptor pair to control vascular development in the retina and inner ear.1 Fz4 expression may play a critical role in responses to Wnt signaling in the tumor microenvironment.2

Antigen Details

NCBI Gene Bank ID

References & Citations

1. Nathans, J. et al. (2007) J Biol Chem. 282: 4057
2. Holcombe, RF. et al. (2007) BMC Cell Biol. 8: 12
IHC FFPE
General Western Blot Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.