E. coli-Derived Monomeric Recombinant Mouse GDF-5 Peptide
<0.1 EU/µg as determined by the LAL method
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
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Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse GDF-5 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit forrmGDF-5 and rmGDF-6 is approximately 5 ng/lane and 25 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Rat Anti-Mouse Growth Differentiation Factor 5 (GDF-5) (Clone 90509) recognizes an epitope on Mouse GDF-5. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Growth differentiation factor 5 (cartilage-derived morphogenetic protein-1), also known as GDF5 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. GDF5 is expressed in the developing central nervous system.1 It increases the survival of neurones that respond to a neurotransmitter called dopamine, and is a potential therapeutic molecule associated with Parkinson's disease.2
NCBI Gene Bank ID
References & Citations
1. Sullivan, A. et al. (2004) J Neurocytol 33: 479
2. O'Keeffe, G. et al. (2005) J Anat 207: 219
Products are for research use only. Not for use in diagnostic or therapeutic procedures.