Anti-Mouse H-2Kd Dd,q,u – PE

Anti-Mouse H-2Kd Dd,q,u – PE

Product No.: H165

[product_table name="All Top" skus="H165"]

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Clone
A5A1-Do4
Target
I H-2Kd Dd
q
u
Formats AvailableView All
Product Type
Monoclonal Antibody
Isotype
IgG3 κ
Applications
FC

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Mouse
Product Concentration
0.2 mg/ml
Formulation
This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This R-PE conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Excitation Laser
Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for clone A5A1-Do4 antibody for staining cells in flow cytometry is ≤ .25 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Class I H-2Kd Dd,q,u (Clone A5A1-Do4) recognizes Class I H-2Kd Dd,q,u. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution
Present on 100% of T-cells, B-cells, erythrocytes, macrophages, endothelial and epithelial tissue. Positive with this antibody are strains of independent haplotypes H-2KdDd,q,u. Positive are intra-H-2 recombinants carrying alleles Kd, Dd, Ld, Dq. Strains carrying all other alleles are negative.

References & Citations

Hauptfeld, V. (1984) Immunogenetics 19:169
Flow Cytometry

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.