Anti-Mouse IgD (Clone 11-26c.2a) – Purified (PhenoCycler-Fusion (CODEX)® Ready)

Clone 11-26c.2a has been validated for use on PhenoCycler® using [Mouse/Human] [Tissue] [Tissue Type either FFPE or FF] tissue.

This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4.

This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

FC,
IF,
IHC,
IP

Clone 11-26c.2a specifically reacts with the delta heavy chain of mouse IgD, without cross- reacting with other classes of mouse immunoglobulin like IgA, IgG, or IgM.

IgD is expressed by peripheral mature B cells.

Immunoglobulin D (IgD) is one of the five main classes of immunoglobulins. Though it is an antibody that is not well understood, it has been discovered to have a vital role in both innate and adaptive immunity. It aids in activating and regulating B cells, especially in the upper respiratory mucosa. In this location, it enhances immune surveillance and defense against respiratory pathogens¹. IgD also helps maintain a healthy microbiome by equipping myeloid effector cells with IgD antibodies that react to mucosal antigens, including both commensal and pathogenic microbes².

The 11-26c.2a clone is an essential tool in immunology research, particularly in the study of B cell development and activation pathways. It is widely used in flow cytometric analysis to identify surface IgD expression on mouse B cells. This provides valuable insights into B cell populations and their roles in immune responses^3,4. When the antibody is conjugated, it allows for easy detection and multiplexing in flow cytometry, enabling comprehensive immunophenotyping studies^5,6.

P01882

1. Chen K, Xu W, Wilson M, et al. Nat Immunol. 2009;10(8):889-898.
2. Gutzeit C, Chen K, Cerutti A. Eur J Immunol. 2018;48(7):1101-1113.
3. Böhmer RM, King NJ. Cytometry. 1984;5(5):543-546.
4. Olejniczak SH, Stewart CC, Donohue K, Czuczman MS. Immunol Invest. 2006;35(1):93-114.
5. Pearson T, Galfrè G, Ziegler A, Milstein C. Eur J Immunol. 1977;7(10):684-690.
6. T B, G K, A K, A F, E U, P N. Journal of immunological methods. 1998;214(1-2).
7. Hamilton AM, Lehuen A, Kearney JF. Int Immunol. 1994;6(3):355-361.
8. Ishihara K, Wood WJ, Wall R, et al. J Immunol. 1993;150(6):2253-2262.
9. Nitschke L, Kosco MH, Köhler G, Lamers MC. Proc Natl Acad Sci U S A. 1993;90(5):1887-1891.
10. Campbell KS, Cambier JC. EMBO J. 1990;9(2):441-448.