Anti-Mouse IL-1R (CD121a) [Clone JAMA-147] — Purified in vivo GOLD™ Functional Grade

Clone JAMA-147 is a hamster monoclonal antibody that specifically recognizes mouse IL-1 receptor type 1 (IL-1R1; CD121a), and it is widely used in in vivo mouse studies to block IL-1–dependent signaling for experimental modulation of the immune response.

Usage in in vivo mouse studies:

• Functional blockade: JAMA-147 is used to block IL-1–dependent cellular responses in mice. This involves administering the antibody to mice to inhibit IL-1R1 signaling, thereby suppressing the biological effects mediated by both IL-1? and IL-1? cytokines. This allows researchers to study the role of the IL-1 pathway in inflammation, immunity, or disease models such as autoimmunity or infection.
• Cellular targets: IL-1R1 is expressed on various mouse cells, including T cells, thymocytes, dendritic cells, fibroblasts, vascular endothelial cells, epithelial cells, and neural cells.
• Experiment applications: The antibody has been cited for use in in vivo IL-1R blockade, meaning it is injected into live mice to interrupt IL-1R1 function during disease modeling or immune response experiments. Best practices include optimizing dosing and scheduling according to experimental design and using the appropriate isotype controls.

Key technical details:

• Isotype: Armenian Hamster IgG, ?.
• Reported applications: In vivo and in vitro blockade of IL-1R1.
• Antibody form: Typically provided unconjugated, suitable for systemic administration.
• Experimental outcomes: Administration of JAMA-147 in vivo leads to inhibition of IL-1–mediated inflammatory responses, allowing researchers to dissect the contribution of this cytokine pathway in various models.

Summary Table: JAMA-147 in vivo mouse application

In summary, clone JAMA-147 is primarily used to block IL-1R1 signaling in live mice, helping to experimentally manipulate and study the effects of the IL-1 pathway in a range of biological contexts.

The correct storage temperature for sterile packaged clone JAMA-147 (anti-mouse IL-1R antibody) is 2–8°C, kept sterile and protected from light.

• According to product information for JAMA-147 antibodies, storage at 2–8°C maintains stability for at least one week. Another source specifies undiluted storage at 4°C in the dark.
• Antibody solutions should not be frozen and must be protected from prolonged light exposure to avoid degradation.

Therefore:

• Do not freeze.
• Store undiluted at 2–8°C (optimally at 4°C) in the dark.

Commonly used antibodies or proteins in the literature alongside JAMA-147 are primarily other monoclonal antibodies targeting distinct epitopes on the type I IL-1 receptor, as well as antibodies against IL-1 itself.

Key co-used antibodies and proteins include:

• JAMA-83
• JAMA-84
• JAMA-110

These antibodies all target the murine type I IL-1 receptor but recognize different epitopes: JAMA-147 targets one, JAMA-84 and JAMA-110 another, and JAMA-83 a third.

• M15 and 35F5: These are other rat monoclonal antibodies specific for the murine type I IL-1 receptor, sometimes compared with or used alongside JAMA-147 for receptor binding and functional studies.

• Anti-IL-1? antibodies

• Anti-type I IL-1 receptor antibodies

These are used in combination (as a pool) for experiments investigating the blockade of IL-1 signaling, both in vitro and in vivo. This combination enables comprehensive inhibition of IL-1-dependent processes.

In summary, studies that utilize JAMA-147 frequently include comparisons or combinations with other JAMA series mAbs (JAMA-83, JAMA-84, JAMA-110), M15, 35F5, and direct anti-IL-1? antibodies to map receptor epitopes, assess blocking efficacy, or study biological outcomes.

The key findings from citations of clone JAMA-147 in scientific literature relate to its application as a biotin-conjugated monoclonal antibody for detecting mouse interleukin-1 receptor type 1 (IL-1R1, also known as CD121a), particularly in immunology studies involving inflammation and antiviral T cell responses.

Essential Findings from Citations

• Functional Use: Clone JAMA-147 is frequently used for flow cytometric identification and quantification of IL-1R1 expression on cell surfaces, especially in mouse models.
• Immunological Insights: Studies employing JAMA-147 have shown that IL-1R1 signaling is crucial for effective antiviral CD8 T cell responses. For example, in models of influenza infection, impaired IL-1 signaling led to deficient effector and tissue-resident CD8 T cell responses, underscoring the receptor’s importance in antiviral immunity.
• Methodological Application: JAMA-147 enables detection and phenotyping of immune cells, often in combination with other cell surface markers (e.g., CD45.1, T-bet, CD44), facilitating detailed mechanistic studies of immune regulation, activation, and inflammation.

Additional Context

• Research Area: JAMA-147 is cited in research spanning innate immunity and inflammatory processes, supporting protocols in both in vitro and in vivo experiments.
• Technical Reliability: Sources identify JAMA-147 as a functional-grade reagent validated for flow cytometry and immunophenotyping in mouse models.

Summary Table

There are no contradictory findings in the cited literature. JAMA-147 is recognized as a standard reagent for mouse IL-1R1 detection, supporting studies of immune mechanisms, especially those involving cytokine signaling and inflammation.