Anti-Mouse IL-1R (CD121a) – Purified in vivo GOLD™ Functional Grade

Pricing & Details

Product No.I-435
Clone
JAMA-147
Protein
Interleukin-1 R
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CD121a
Isotype
Armenian Hamster
IgG
Applications
B
,
IP
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
I-435-1.0 mg
1.0 mg
$98.00
In stock
Max:
Min: 1
Step: 1
I-435-5.0 mg
5.0 mg
$325.00
In stock
Max:
Min: 1
Step: 1
I-435-25 mg
25 mg
$875.00
In stock
Max:
Min: 1
Step: 1
I-435-50 mg
50 mg
$1,450.00
In stock
Max:
Min: 1
Step: 1
I-435-100 mg
100 mg
$2,125.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Armenian Hamster
Immunogen
Extracellular domain of mouse IL-1 R type 1
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day Ambient
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Armenian Hamster Anti-Mouse IL-1 R1 (Clone JAMA-147) recognizes Mouse IL-1 R1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
IL-1 binds two receptors, IL-1RI and IL-1RII. The ligands IL-1α and IL-1ß exert all of their various biological actions by binding IL-1RI. IL-1RII has a shortened cytoplasmic domain that is unable to transduce signals and therefore acts as a decoy receptor and competitively antagonizes the binding of IL-1α and IL-1ß, thereby reducing their biological effects. Binding of the ligands to IL-1RI is enhanced by an accessory protein, IL-1R-AcP. IL-1RI is expressed on T cells, thymocytes, dendritic cells, fibroblasts, vascular endothelial cells, epithelial cells and neural cells. It is an 80 kD transmembrane glycoprotein.

Antigen Details

Ligand/Receptor
IL-1α and IL-1β
NCBI Gene Bank ID
Research Area
Immunology
.
Innate Immunity
Immunoprecipitation Protocol
General Western Blot Protocol