Anti-Mouse IL-4 R

Anti-Mouse IL-4 R

Product No.: I-722

[product_table name="All Top" skus="I-722"]

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Target
IL-4 R
Product Type
Polyclonal Antibody
Alternate Names
Interleukin-4 Receptor, CD124
Applications
CyTOF®
,
FC
,
WB

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Select Product Size
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Goat
Immunogen
NS0 - Derived Recombinant Mouse Interleukin-4 Receptor Extracellular Domain
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing IL-4 R. A suggested method would be to stain cells expressing IL-4 R with 2.5 per 1 x 106 cells in a total staining volume of 100 µl followed by PN:G641.
Western Blotting: To detect Mouse IL-4 R this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse IL-4 R is 0.5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Additional Applications Reported In Literature ?
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Goat Anti-Mouse Interleukin 4 Receptor (IL-4 R) recognizes Mouse IL-4 R. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
IL-4 can signal through type I and type II receptor complexes, which share a common gamma chain. The type I receptor contains in addition to the gamma chain an IL-4R alpha subunit, whereas the type II receptor contains the IL-13R alpha. The secreted extracellular domain of IL-4R alpha, called sIL-4R alpha, binds IL-4 and antagonizes its activity. It plays an important role in regulating the differentiation of naive CD4+ T cells and class switching to IgG1 and IgE.
PubMed
NCBI Gene Bank ID

References & Citations

CyTOF®
Flow Cytometry
General Western Blot Protocol

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.