Anti-Mouse IL-4 R (Clone 129801)

Anti-Mouse IL-4 R (Clone 129801)

Product No.: I-676

[product_table name="All Top" skus="I-676"]

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Clone
129801
Target
IL-4 R
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CD124, Interleukin-4 Receptor
Isotype
IgG2b
Applications
WB

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
NS0-Derived Recombinant Mouse Interleukin-4 Receptor Extracellular Domain
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse IL-4 R this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse IL-4 R is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse Interleukin 4 Receptor (IL-4 R) (Clone 129801) recognizes an epitope on Mouse IL-4 R. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows less than 1% cross-reactivity with rhIL-4 R.
Background
IL-4 can signal through type I and type II receptor complexes, which share a common gamma chain. The type I receptor contains in addition to the gamma chain an IL-4R alpha subunit, whereas the type II receptor contains the IL-13R alpha. The secreted extracellular domain of IL-4R alpha, called sIL-4R alpha, binds IL-4 and antagonizes its activity. It plays an important role in regulating the differentiation of naive CD4+ T cells and class switching to IgG1 and IgE.

Antigen Details

PubMed
NCBI Gene Bank ID
General Western Blot Protocol

Certificate of Analysis

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Prod No.
Description
I-722
I-782

Formats Available

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Prod No.
Description
I-676
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.