Anti-Mouse Ly-6G – DyLight® 488

Anti-Mouse Ly-6G – DyLight® 488

Product No.: L287

[product_table name="All Top" skus="L287"]

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Clone
1A8
Target
Ly-6G
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Lymphocyte Antigen 6 Complex, Ly6G
Isotype
IgG2a
Applications
FC
,
IF Staining

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Product Size

Data

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
Purified Recombinant Mouse Ly-6G (>98%)
Product Concentration
0.2 mg/ml
Formulation
This DyLight® 488 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This DyLight® 488 conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Excitation Laser
Blue Laser (493 nm)
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 1A8 recognizes an epitope on mouse Ly6G. Clone 1A8 does not cross react with Ly6C.
Antigen Distribution
Ly6G is expressed by neutrophils.
Background
Ly6G antibody, clone 1A8, recognizes lymphocyte antigen 6 complex locus G6D (Ly6G; also called Gr-1), a 21-25 kDa glycosylphosphatidylinositol (GPI)-anchored protein1. Ly6G belongs to the lymphocyte antigen-6 (Ly6)/urokinase-type plasminogen activator receptor (uPAR) superfamily, characterized by a Ly6/uPAR (LU) domain-containing a three-fingered structural motif stabilized by disulfide bonds2. Ly6G is expressed by murine neutrophils regardless of location and activation1,4,5. Eosinophils may also express low levels of Ly6G5. There is no human ortholog for Ly6G; however, a structurally related L76/uPAR protein, CD177 (also known as HNA-2a, NB1, or PRV-1) is expressed in human neutrophils and is implicated in neutropenia6. Although the exact function and ligand of Ly6G remain unknown, Ly6G ligation may impair neutrophil migration to sites of inflammation via a β2-integrin-dependent mechanism7.

Antigen Details

PubMed
NCBI Gene Bank ID
Research Area
Immunology
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Innate Immunity

References & Citations

1.) Fleming TJ, et al. (1993) J Immunol. 151(5):2399-408
2.) Tsetlin VI. et al. (2015) Trends Pharmacol Sci. 36(2):109-23
3.) Daley JM, et al. (2008) J Leukoc Biol. 83(1):64-70
4.) Lee PY, et al. (2013) J Leukoc Biol. 94(4):585-594
5.) Percopo CM, et al. (2017) J Leukoc Biol. 101(1):321-328.
6.) Stroncek DF. et al. (2007) Curr Opin Hematol. 14(6):688-93
7.) Wang JX, et al. (2012) Blood. 120(7):1489-1498
8.) Uwki, H. et al. (2020) Nature Protocols 15:1041–1065 Journal Link
Flow Cytometry
IF Staining

Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.