Anti-Mouse Ly-6G – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Anti-Mouse Ly-6G – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Product No.: L500

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Clone
1A8
Target
Ly-6G
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Lymphocyte Antigen 6 Complex
Isotype
IgG2a
Applications
IHC FF
,
PhenoCycler®

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Product Size
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CODEX® Details

Validation Notes
Clone 1A8 has been validated for use on CODEX® using mouse spleen fresh frozen (FF) tissue.
Tissue Screened
Spleen
Tissue Preparation
FF (Fresh Frozen)

Antibody and Reporter Details

Reactivity Species
Mouse
Host Species
Rat
Concentration
0.5 mg/ml
Immunogen
Ly-6G-transfected EL4J Cell Line
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4.
Storage and Handling
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Applications and Recommended Usage?
Quality Tested by Leinco
CODEX® This Ly-6G (Clone 1A8) antibody is formulated to simplify the antibody preparation needed when performing a CODEX® barcode conjugate. The suggested concentration is 0.5 mg/ml.
RRID
AB_2893871
Country of Origin
USA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 1A8 recognizes an epitope on mouse Ly6G. Clone 1A8 does not cross react with Ly6C.
Antigen Distribution
Ly6G is expressed by neutrophils.
Background
Ly6G antibody, clone 1A8, recognizes lymphocyte antigen 6 complex locus G6D (Ly6G; also called Gr-1), a 21-25 kDa glycosylphosphatidylinositol (GPI)-anchored protein1. Ly6G belongs to the lymphocyte antigen-6 (Ly6)/urokinase-type plasminogen activator receptor (uPAR) superfamily, characterized by a Ly6/uPAR (LU) domain-containing a three-fingered structural motif stabilized by disulfide bonds2. Ly6G is expressed by murine neutrophils regardless of location and activation1,4,5. Eosinophils may also express low levels of Ly6G5. There is no human ortholog for Ly6G; however, a structurally related L76/uPAR protein, CD177 (also known as HNA-2a, NB1, or PRV-1) is expressed in human neutrophils and is implicated in neutropenia6. Although the exact function and ligand of Ly6G remain unknown, Ly6G ligation may impair neutrophil migration to sites of inflammation via a β2-integrin-dependent mechanism7.

Antigen Details

Protein
PubMed
NCBI Gene Bank ID

References & Citations

1. Fleming TJ, at al. (1993) J Immunol. 151(5):2399-408
2. Tsetlin VI. (2015) Trends Pharmacol Sci. 36(2):109-23
3. Daley JM, et al. (2008) J Leukoc Biol. 83(1):64-70
4. Lee PY, et al. (2013) J Leukoc Biol. 94(4):585-594
5. Percopo CM, et al. (2017) J Leukoc Biol. 101(1):321-328.
6. Stroncek DF. (2007) Curr Opin Hematol. 14(6):688-93
7. Wang JX, et al. (2012) Blood. 120(7):1489-1498
IHC
PhenoCycler®
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Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.