Anti-Mouse Ly-6G – Purified In vivo GOLD™ Functional Grade

Pricing & Details

  
Clone
1A8
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Lymphocyte antigen 6 complex, locus G
Isotype
Rat
IgG2a κ
Applications
CODEX®
,
CyTOF®
,
Depletion
,
FC
,
IHC FF
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
L280-1.0 mg
1.0 mg
$95.00
In stock
L280-5.0 mg
5.0 mg
$298.00
In stock
L280-25 mg
25 mg
$775.00
In stock
L280-50 mg
50 mg
$1,275.00
In stock
L280-100 mg
100 mg
$1,998.00
In stock

Data

  

Antibody Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
Mouse Ly-6G transfected EL-4J cell line
Product Concentration
≥ 2.0 mg/ml
1.0 mg/ml
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Purity
>95% by SDS-PAGE and HPLC
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Applications and Recommended Usage ?
(Quality Tested by Leinco)
FC The suggested concentration for this AFS98 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Other Applications Reported In Literature ?
CyTOF®
Depletion
IHC (Frozen)
IHC (Paraffin)
WB
Each investigator should determine their own optimal working dilution for specific applications.

Description

Specificity
Rat Anti-Mouse Ly-6G (Clone 1A8) recognizes an epitope on Mouse Ly-6G. Unlike clone RB6-8C5 antibody, the 1A8 antibody reacts specifically with mouse Ly6G with no reported cross-reactivity with Ly6C. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution
Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.
Background
Ly-6G is a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Antigen Details

Protein
Ly-6G
PubMed
Ly-6G+Mouse+1A8
NCBI Gene Bank ID

References & Citations

1. Fleming, TJ. et al. (1993) J. Immunol.151:2399.
Flow Cytometry
IHC
General Western Blot Protocol
  

Formats Available

  
Products are for research use only. Not for use in diagnostic or therapeutic procedures.