Anti-Mouse MCP-5

Product No.: M149


Target MCP-5 Product Type Polyclonal Antibody Alternate Names SCYA12, CCL12, Monocyte Chemoattractant Protein-5 Applications N , WB


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Antibody Details Product Details Reactive Species Mouse Host Species Goat Immunogen Purified Recombinant Mouse MCP-5 (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2831353 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse MCP-5 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R1190 and a chromogenic substrate such as PN:T343. The detection limit for Mouse MCP-5 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M163. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Mouse MCP-5 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Mouse MCP-5 (at 0.2 ng/ml) is 0.8-4 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity Goat Anti-Mouse Monocyte Chemotactic Protein 5 (MCP-5) recognizes Mouse MCP-5. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. PubMed MCP-5 NCBI Gene Bank ID 20293 UniProt.org More Information on Uniprot.org References & Citations 1. Luster, AD. et al. (1997) J Exp Med. 185: 99 2. Jia, GQ. et al. (1996) J Exp Med. 184: 1939 View product citations for antibody M149 on CiteAb Technical Protocols N Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Mouse

Host Species

Goat

Immunogen

Purified Recombinant Mouse MCP-5 (>98%)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

State of Matter

Lyophilized

Storage and Handling

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day 2-8°C

RRIDAB_2831353

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Mouse MCP-5 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R1190 and a chromogenic substrate such as PN:T343. The detection limit for Mouse MCP-5 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M163.

Additional Applications Reported In Literature ?

Neutralization: This antibody is useful for neutralization of Mouse MCP-5 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Mouse MCP-5 (at 0.2 ng/ml) is 0.8-4 µg/ml.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Mouse Monocyte Chemotactic Protein 5 (MCP-5) recognizes Mouse MCP-5. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

PubMed

MCP-5

NCBI Gene Bank ID

20293

UniProt.org

More Information on Uniprot.org