Anti-Mouse NK1.1 [Clone PK136] — Purified in vivo PLATINUM™ Functional Grade
Anti-Mouse NK1.1 [Clone PK136] — Purified in vivo PLATINUM™ Functional Grade
Product No.: N268
Clone PK136 Target NK1.1 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Natural Killer Cells, KR-P1C, NKR-P1B, Ly-55, CD161b, CD161c Isotype Mouse IgG2a k Applications B , CyTOF® , Depletion , FC , in vivo , IP , WB |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Mouse Recommended Isotype Controls Recommended Dilution Buffer Immunogen NK-1+ cells from mouse spleen and bone marrow Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2831485 Additional Applications Reported In Literature ? CyTOF® Depletion IP WB B Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone PK136 recognizes mouse NK1.1. Background NK1.1 is a type II membrane protein that is part of the C-type lectin superfamily. NK1.1 contains a transmembrane domain and a cytoplasmic domain that are characteristic of C-type lectins. The function of NK cells is to mediate cytotoxicity and to secrete cytokines after immune stimulation. NK1.1 has been correlated with lysis of tumor cells In vitro and rejection of bone marrow allografts In vivo. It is also involved in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 is commonly used as a mouse NK cell marker. Antigen Distribution NK-1.1 is encoded by the NKR-P1B/NKR-P1C gene and expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. PubMed NCBI Gene Bank ID UniProt.org Research Area Immunology . Innate Immunity Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone PK136 is primarily used in vivo for the depletion of natural killer (NK) cells in mice to study their roles in immunity, cancer, and infection. Beyond NK cell depletion, it is also utilized for blocking/neutralizing NK1.1 function, flow cytometry, and functional assays. Key in vivo applications include:
Summary Table of Common In Vivo Applications of PK136 in Mice
In summary, PK136’s most common in vivo use is for targeted depletion of NK cells, with additional applications in blocking, phenotyping, and functional studies in mouse models where NK1.1 is expressed. Proper mouse strain selection is essential for effective use. Commonly used antibodies or proteins used alongside PK136 in the literature include anti-CD3ε, anti-CD49b (DX5), and anti-Asialo GM1 for mouse natural killer (NK) cell identification and depletion studies. These antibodies are frequently chosen due to their ability to complement PK136, facilitating the distinction or functional manipulation of NK cells in various settings. Essential context and supporting details:
Additional relevant antibodies and controls:
These combinations optimize identification, depletion, or functional manipulation of NK cells for in vivo and in vitro studies across different experimental contexts and mouse strains. Clone PK136 is a monoclonal antibody widely cited in scientific literature for its high specificity to the mouse NK1.1 antigen, a cell surface marker primarily found on natural killer (NK) cells and certain T cell subsets. The key findings from studies using clone PK136 are as follows:
In sum, PK136 is foundational for studying NK cell biology in vivo in NK1.1-expressing mouse strains, with findings broadly supporting its specificity, efficiency for cell identification and depletion, and critical role in advancing our understanding of NK cell function in immunity and disease. Dosing regimens of the anti-NK1.1 antibody clone PK136 vary across mouse models primarily by dose, frequency, and duration, depending largely on the mouse strain and experimental goals.
Key variables impacting regimen selection:
In summary, PK136 dosing in mouse models often involves intraperitoneal injection of 200–500 μg per mouse per dose, singly or on a repeated schedule, with optimization based on mouse genetics and experimental design. References & Citations1. Ardolino, M. et al. (2018) J Clin Invest. 128(10):4654-4668. PubMed 2. Koo, GC. and Peppard, JR. (1986) Hybridoma 3:301 Technical ProtocolsCertificate of Analysis |
Formats Available
Prod No. | Description |
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N228 | |
N106 | |
N109 | |
N108 | |
N107 | |
N110 | |
N220 | |
N221 | |
N222 | |
N224 | |
N226 | |
N123 | |
N268 |
