Anti-Mouse NK1.1 – Purified In vivo GOLD™ Functional Grade

Pricing & Details

Product No.N123
Clone
PK136
Protein
NK1.1
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Natural Killer Cells, KR-P1C, NKR-P1B, Ly-55, CD161b, CD161c
Isotype
Mouse
IgG2a κ
Applications
B
,
CyTOF®
,
Depletion
,
FC
,
IP
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
N123-1.0 mg
1.0 mg
$95.00
In stock
Max:
Min: 1
Step: 1
N123-5.0 mg
5.0 mg
$298.00
In stock
Max:
Min: 1
Step: 1
N123-25 mg
25 mg
$800.00
In stock
Max:
Min: 1
Step: 1
N123-50 mg
50 mg
$1,325.00
In stock
Max:
Min: 1
Step: 1
N123-100 mg
100 mg
$1,998.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Mouse
Immunogen
NK-1+ cells from mouse spleen and bone marrow
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<1.0 EU/µg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Applications and Recommended Usage?
Quality Tested by Leinco
Flow Cytometry: The suggested use of this antibody is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Other Applications Reported In Literature ?
CyTOF® (Validated)
IP
Depletion
Block
IHC
IF (Reported in the literature)
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone PK136 recognizes mouse NK1.1.
Antigen Distribution
NK-1.1 is encoded by the NKR-P1B/NKR-P1C gene and expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129.
Background
NK1.1 is a type II membrane protein that is part of the C-type lectin superfamily. NK1.1 contains a transmembrane domain and a cytoplasmic domain that are characteristic of C-type lectins. The function of NK cells is to mediate cytotoxicity and to secrete cytokines after immune stimulation. NK1.1 has been correlated with lysis of tumor cells In vitro and rejection of bone marrow allografts In vivo. It is also involved in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 is commonly used as a mouse NK cell marker.

Antigen Details

Protein
Function
NK cell activation, IFN-γ production, cytotoxic granule release
PubMed
NCBI Gene Bank ID
Research Area
Immunology
.
Innate Immunity

References & Citations

1.) Ardolino, M. et al. (2018) J Clin Invest. 128(10):4654-4668. PubMed
2.) Koo, GC. and Peppard, JR. (1986) Hybridoma 3:301
3.) Raju et al. (2019) Cell Reports. 29:2556–2564 Journal Link
Flow Cytometry
Immunoprecipitation Protocol
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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