Anti-Mouse NK1.1 – Purified in vivo GOLD™ Functional Grade
Pricing & Details
NK-1+ cells from mouse spleen and bone marrow
≥ 5.0 mg/ml
< 1.0 EU/mg as determined by the LAL method
≥95% monomer by analytical SEC
>95% by SDS Page
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
Flow Cytometry: The suggested use of this antibody is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Other Applications Reported In Literature ?
IF (Reported in the literature)
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Clone PK136 recognizes mouse NK1.1.
NK-1.1 is encoded by the NKR-P1B/NKR-P1C gene and expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129.
NK1.1 is a type II membrane protein that is part of the C-type lectin superfamily. NK1.1 contains a transmembrane domain and a cytoplasmic domain that are characteristic of C-type lectins. The function of NK cells is to mediate cytotoxicity and to secrete cytokines after immune stimulation. NK1.1 has been correlated with lysis of tumor cells In vitro and rejection of bone marrow allografts In vivo. It is also involved in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 is commonly used as a mouse NK cell marker.
NK cell activation, IFN-γ production, cytotoxic granule release
NCBI Gene Bank ID
References & Citations
1.) Ardolino, M. et al. (2018) J Clin Invest. 128(10):4654-4668. PubMed
2.) Koo, GC. and Peppard, JR. (1986) Hybridoma 3:301
3.) Raju et al. (2019) Cell Reports. 29:2556–2564 Journal Link