Anti-Mouse NK1.1 Antibody – Purified in vivo GOLD™ Functional Grade

Anti-Mouse NK1.1 Antibody – Purified in vivo GOLD™ Functional Grade

Product No.: N123

[product_table name="All Top" skus="N123"]

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Clone
PK136
Target
NK1.1
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Natural Killer Cells, KR-P1C, NKR-P1B, Ly-55, CD161b, CD161c
Isotype
IgG2a κ
Applications
B
,
CyTOF®
,
Depletion
,
FC
,
in vivo
,
IP
,
WB

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Product Size
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Mouse
Immunogen
NK-1+ cells from mouse spleen and bone marrow
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal NK1.1 antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
Flow Cytometry: The suggested use of this NK1.1 antibody is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Applications Reported In Literature ?
CyTOF® (Validated)
IP
Depletion
Block
IHC
IF (Reported in the literature)
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
This NK1.1 antibody (clone PK136) recognizes mouse NK1.1.
Antigen Distribution
NK-1.1 is encoded by the NKR-P1B/NKR-P1C gene and expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129.
Background
NK1.1 is a type II membrane protein that is part of the C-type lectin superfamily. NK1.1 contains a transmembrane domain and a cytoplasmic domain that are characteristic of C-type lectins. The function of NK cells is to mediate cytotoxicity and to secrete cytokines after immune stimulation. NK1.1 has been correlated with lysis of tumor cells In vitro and rejection of bone marrow allografts In vivo. It is also involved in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 is commonly used as a mouse NK cell marker.

Antigen Details

Function
NK cell activation, IFN-γ production, cytotoxic granule release
PubMed
NCBI Gene Bank ID
Research Area
Immunology
.
Innate Immunity

References & Citations

1.) Ardolino, M. et al. (2018) J Clin Invest. 128(10):4654-4668. PubMed
2.) Koo, GC. and Peppard, JR. (1986) Hybridoma 3:301
3.) Raju et al. (2019) Cell Reports. 29:2556–2564 Journal Link
B
CyTOF®
Depletion
Flow Cytometry
in vivo Protocol
Immunoprecipitation Protocol
General Western Blot Protocol
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Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.