Anti-Mouse PD-L2 (B7-DC) (Clone TY25) – Purified in vivo PLATINUM™ Functional Grade

The clone TY25 is used in in vivo mouse studies primarily as an anti-mouse PD-L2 (B7-DC) antibody. It is utilized to block PD-L2 interactions with its receptor, PD-1, which are involved in inhibiting T cell activation and proliferation. This blockade is crucial in understanding the role of PD-L2 in immune regulation and its potential impact on cancer immunotherapy. The TY25 antibody is specifically engineered for in vivo applications, often in mouse models of cancer or immune-related diseases, to study the effects of blocking the PD-1/PD-L2 interaction on immune responses and disease outcomes.

In these studies, the TY25 antibody is usually administered to mice to evaluate its ability to enhance immune responses by disrupting the PD-1/PD-L2 inhibitory pathway. By blocking PD-L2, TY25 can potentially increase T cell activation, leading to improved antitumor responses or modulation of immune responses in experimental autoimmune models.

The antibody is available in a mouse IgG2a format, which is suitable for in vivo experiments, allowing researchers to study its effects in a biologically relevant context.

I cannot find specific storage temperature information for the sterile packaged clone TY25 in the provided search results. The search results contain general storage guidelines for various biological materials like competent cells, DNA fragments, vaccines, and cell cultures, but none specifically mention clone TY25.

Without access to the specific product information or manufacturer's guidelines for TY25, I cannot provide the correct storage temperature for this particular clone. To obtain accurate storage instructions, you would need to:

• Check the product documentation or packaging that came with TY25
• Contact the manufacturer or supplier directly
• Review the product datasheet or technical specifications from the vendor
• Consult any certificates of analysis or quality control documents provided with the product

Different types of biological materials have vastly different storage requirements - from room temperature to -80°C or even liquid nitrogen temperatures - so it's crucial to follow the specific guidelines for your particular product to maintain its viability and integrity.

Commonly used antibodies or proteins in the literature alongside TY25 include other markers of immune checkpoint pathways, such as PD-L1 (CD274), PD-1, and costimulatory molecules, as well as isotype controls and functional antibodies for immune cell activation and inhibition assays.

The TY25 antibody specifically recognizes mouse CD273 (B7-DC, PD-L2), a ligand in the programmed death pathway. Literature frequently pairs TY25 with these antibodies:

• Anti-PD-1: PD-1 is the receptor for PD-L2 and PD-L1; anti-PD-1 antibodies help evaluate the functional interaction and blockade of this immunoregulatory axis.
• Anti-PD-L1 (CD274): Used parallelly to distinguish the roles of the two PD-1 ligands in immune modulation.
• Isotype controls: Employed in flow cytometry and functional experiments to control for nonspecific antibody effects.
• T cell markers (CD3, CD4, CD8): These are used to identify and distinguish T cell populations during immune cell phenotyping, often in conjunction with TY25 when analyzing PD-L2 expression on antigen-presenting cells or measuring effects on T cell activation.
• Costimulatory or coinhibitory molecules (e.g., CD80, CD86): As PD-L2 participates in broader immunoregulatory networks, antibodies for related costimulatory molecules are commonly included.

In functional and binding studies, TY25 might also be used along with recombinant fusion proteins such as PD-1-Fc, which serve as receptor ligands to study binding specificity and competition dynamics with endogenous PD-L2. Additionally, in cell-based systems, positive controls such as anti-leucine zipper antibodies or fusion proteins are used, especially in systems like Bacterial Adenylate Cyclase Two-Hybrid (BACTH) assays (though TY25 itself is not directly referenced in BACTH literature).

Summary of commonly paired antibodies/proteins:

• Anti-PD-1
• Anti-PD-L1 (CD274)
• Isotype controls
• T cell population markers (CD3, CD4, CD8)
• Costimulatory/coinhibitory molecules (CD80, CD86)
• PD-1-Fc fusion proteins

These combinations allow for detailed investigation of the PD-1/PD-L2/PD-L1 immune pathway, facilitating studies of checkpoint blockade, T cell activation, and immune evasion in cancer and infectious disease models.

Scientific literature citing "clone TY25" primarily references a well-characterized human CD25+CD4+ T cell suppressor clone. The key findings associated with clone TY25 and related CD25+CD4+ clones include:

• Heterogeneity of Human CD25+CD4+ T Cells: Cloned CD25+CD4+ T cells are not uniform; only a subset, such as TY25, continue to express high levels of CD25 and exhibit suppressive functions.
• Cloning and Characterization Methods: Clone TY25 was isolated using fluorescence-activated cell sorting (FACS) from peripheral blood, achieving high purity (over 98%). Cloning employed limiting dilution and restimulation protocols, ensuring clonality and functional consistency.
• Functional Properties: TY25 and other suppressive clones display strong suppression of other T cell proliferation. These clones produce immunoregulatory cytokines and are unresponsive to certain polyclonal stimuli unless supplemented with exogenous interleukin-2 (IL-2).
• T Cell Receptor (TCR) Uniqueness: Each CD25+CD4+ suppressor clone, including TY25, expresses a single TCR V? chain, confirming monoclonality and suggesting antigen-specific regulatory potential.
• Biological Role: TY25 and similar clones illustrate mechanisms by which regulatory T cells (Tregs) control immune responses, with implications for autoimmunity, transplantation, and immunotherapy.

No references in scientific literature link "clone TY25" to yeast Ty1 elements or plant Ty1/copia retrotransposons; those are unrelated uses of similar nomenclature.

In summary, clone TY25 is foundational in defining the properties of human CD25+CD4+ regulatory T cells, highlighting their suppressive capabilities, unique TCR usage, and methodological advancements in their isolation and study.