Anti-Mouse PD-L2 (B7-DC) – Purified In vivo GOLD™ Functional Grade

Pricing & Details

Product No.C2292
Clone
TY25
Protein
PD-L2
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
PD-L2 , CD273, B7-DC
Isotype
Rat
IgG2a κ
Applications
FC
,
IHC FF
,
IP
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C2292-1.0 mg
1.0 mg
$95.00
In stock
Max:
Min: 1
Step: 1
C2292-5.0 mg
5.0 mg
$298.00
In stock
Max:
Min: 1
Step: 1
C2292-25 mg
25 mg
$775.00
In stock
Max:
Min: 1
Step: 1
C2292-50 mg
50 mg
$1,275.00
In stock
Max:
Min: 1
Step: 1
C2292-100 mg
100 mg
$1,998.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
Purified transfected mouse B7-DC cell line
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this CD273 antibody clone TY25 for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
WB
Other Applications Reported In Literature ?
IHC FF
IP
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone TY25 recognizes an epitope on mouse PD-L2. TY25 does not cross-react with PD-L1.
Antigen Distribution
PD-L2 is expressed on macrophages and a subset of dendritic cells (DCs).
Background
PD-L2 antibody, clone TY25, recognizes mouse programmed death ligand 2 (PDL-2), also known as B7-DC and CD273. PD-L2 is a 42 kDa type I transmembrane glycoprotein that belongs to the B7 family of the immunoglobulin (Ig) receptor superfamily. PD-L2 is an inhibitory ligand primarily expressed on antigen-presenting cells, including macrophages and a subset of dendritic cells (DCs). Its receptor, PD-1, is expressed by CD4-CD8- thymocytes as well as CD4 and CD8 T cells, monocytes, DCs, and B cells upon activation1. Binding of PD-L2 to PD-1 on CD4 and CD8 T cells inhibits T cell receptor (TCR) signaling, negatively regulating T cell proliferation, cytokine production, and cytotoxic activity2. PD-L2 is expressed on many tumor types3,4 and is associated with unfavorable prognosis in patients with solid tumors5.

Antigen Details

Protein
Ligand/Receptor
PD-1, uncharacterized receptor
PubMed
NCBI Gene Bank ID
Research Area
Costimulatory Molecules
.
Immunology

References & Citations

1.) Francisco LM, et al. (2010) Immunol Rev. 236:219-242
2.) Latchman Y, et al. (2001) Nat Immunol. 2(3):261-8
3.) Ahmad SM, et al. (2018) Oncoimmunology. 7:e1390641
4.) Panjwani PK, et al. (2018) Hum Pathol. 71:91–9
5.) Yang H, et al. (2019) Front Oncol. 9:47
6.) Gubin et al. (2018) Cell. 175:1014–1030 Journal Link
Flow Cytometry
IHC
Immunoprecipitation Protocol
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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